Liquid Chromatography Mass Spectrometry System for Shotgun Proteomics and Immunoproteomics

拟议的仪器是一个高分辨率液相色谱质谱（LC-MS）系统，结合了各种技术，允许多功能的应用。首先，该仪器将使汉诺威兽医大学（TiHo）基金会的生物化学和感染生物学研究人员能够对细胞培养和组织样本进行高灵敏度的定量蛋白质和聚糖分析。特别是，研究病原体-宿主相互作用的感染学家和阐明致病机制的药理学家将从该技术中受益。多种应用包括感染或药物干预后蛋白质的定量表达分析、感染或神经元疾病期间蛋白质相互作用/蛋白质复合物的分析、感染背景下聚糖的表征、免疫细胞呈递的表位的鉴定、翻译后修饰的分析以及神经病理蛋白质型的自上而下分析。此次仪器收购将在TiHo汉诺威建立一个新的、可广泛使用的质谱核心，并使生物化学研究所能够使用最先进的技术解决传染病中的蛋白质和聚糖分析问题。

The proposed instrument is a high-resolution liquid chromatography mass spectrometry (LC-MS) system that combines various techniques to allow versatile applications. Primarily, the instrument will enable biochemistry and infection biology researchers at the University of Veterinary Medicine Hannover (TiHo) Foundation to perform highly sensitive quantitative protein and glycan analyses of cell culture and tissue samples. In particular, infectiologists studying pathogen-host interactions and pharmacologists illuminating mechanisms of pathogenicity will benefit from the technology. Multiple applications include quantitative expression analysis of proteins following infection or pharmacological intervention, analysis of protein interactions/protein complexes during infection or neuronal disease, characterization of glycans in the context of infection, identification of epitopes presented by immune cells, analysis of post-translational modifications, and and top down analysis of neuropathological proteoforms. The instrument aqcuisition will establish a new, broadly accessible mass spectrometry core at TiHo Hannover and enable the Institute for Biochemistry to address protein and glycan analytical questions in infectious diseases using state-of-the-art technology.