Identification of aging-related factors that facilitate Alzheimer's disease

1.我们鉴定GLIPICAN-1是一种Aβ结合蛋白。Glypcan-1富含RAFT组分，并沉积在衰老斑块中。2.通过对β野生型和突变型细胞系的差异显示，我们鉴定了一种新的蛋白质--腺泡素。ADOPLIN是一种膜蛋白，与PS1形成复合体。利用酵母双杂交系统，我们鉴定了一个新的Aβ结合蛋白AB-DIP，该蛋白含有CARD和核靶向序列。AB-DIP的过度表达诱导细胞死亡，增强Aβ诱导的细胞死亡。4.Neprilysin基因敲除小鼠与突变的pS1转基因小鼠杂交。小鼠没有表现出阿尔茨海默病表型的增强效应。

1.We identified glypican-1 as an Aβ-binding protein. Glypican-1 was enriched in the raft fraction, and deposited in senile plaques. Glypican-1 enhanced Aβ-induced cell death.2.By using the differential display between PS1 wild and mutant cell lines, we identified a novel protein, adoplin. Adoplin was a membrane protein, and made a complex with PS1. Over expression of adoplin enhanced Aβ production.3.By using the yeast two-hybrid system, we identified a novel Aβ-binding protein, AB-DIP, which contained CARD and nuclear targeting sequence. Overe-xpression of AB-DIP itself induced cell death and enhanced Aβ- induced cell death.4.Neprilysin-knock-out mice were crossed with mutant PS1-transgenic mice. The mice did not show an enhancing effect of the Alzheimer phenotype.