Search for antitumor marine natural products through growth recovery assay in engineered yeasts

在天然产物的发现研究中，最重要的问题是如何将高通量的体外分析结果与基于细胞的体内活性联系起来。吉田博士构建的过量表达致死基因的突变酵母的生长恢复分析是实现这一目的的一种选择方法。从海洋无脊椎动物的提取物中，我们从海洋海绵Theonella swinhoei中分离出两种截短的硫胺素衍生物，并从一种身份不明的海绵中分离出三种类固醇糖苷。

Most important issue in the natural products discovery study is how to link the high-through-put in vitro assay results and cell-based in vivo activities. The growth recovery assay of the otherwise lethal gene-overexpressing mutant yeasts constructed by Dr. Yoshida is a method of choice for such purposes. From the extracts of marine invertebrates we isolated two truncated thiamine derivatives from the marine sponge Theonella swinhoei and three steroid glycosides from an unidentified marine sponge.