Methodological studies for the control of three invasive alien ant species using molecular-biological techniques, sterile males and natural enemies

我们证实阿根廷蚂蚁正在沿着日本太平洋沿岸地区扩大其分布，尽管我们使用Z-（9）-十六碳烯醛以及一种常见的杀蚁剂成功地消灭了横滨的一个小种群。为了防止红火蚁入侵日本，我们开发了识别（使用DNA条形码）和检测（使用寄生蜂Microplitis croceipes）的方法，这些方法甚至可以由检疫官员轻松使用。在台湾、美国和阿根廷的调查中，我们发现在RIRA最近入侵的国家，多倍体工人迅速增加。利用分子生物学中的一种重要工具Diacamma sp.研究了蚂蚁的性别决定机制，发现了一些有趣的基因表达模式，如雌性化基因。

We confirmed that Argentine ants are expanding their distribution along the coastal areas of Pacific Ocean side in Japan, though we succeeded in eradication of one small population in Yokohama using Z-(9)-Hexadecenal as well as a common myrmecocide. To prevent the invasion of RIFA into Japan, we developed methods for identification (using DNA bar coding) and detection (using the parasitoid wasp Microplitis croceipes) which can readily be used even by quarantine officers. In the surveys in Taiwan, USA and Argentina, we found that polyploid workers rapidly increased in the countries where RIRA had recently invaded. Using Diacamma sp. which is useful for molecular biology, we studied the sex determination mechanism in ants and found some intriguing expression patterns of genes such as feminizer.