Construction of Gene network for panicle development based on trait and expression QTL anaylisis.

利用籼稻品种Habataki与粳稻Koshihikari杂交的83个bill s，研究了水稻穗部发育的分子机制。在QTL分析中，我们对每穗穗数、穗长等34个性状（性状QTL）进行了评价。我们还通过微阵列分析进行表达QTL分析（eQTL），分析了所有水稻基因的表达水平。通过性状QTL分析，鉴定出83个与穗部结构有关的QTL。此外，我们建议将性状-QTL和eQTL数据进行比较分析，以预测QTL候选基因的有效性。首先，我们对OsCKX2/Gn1a进行了定位克隆，并成功预测了最可行的Gn1a位点候选位点。使用相同的方法，我们成功地预测了qPBL-6、qPBL-7和pUPB-8的几个候选基因。

We investigated the molecular mechanism of rice panicle development by using 83 BILs crossed between an indica variety, Habataki, and Japonica, Koshihikari. For QTL analysis, we evaluated 34 traits, such as panicle number per panicle, the length of panicle, , etc (traitQTL). We also analyzed the expression level of all rice genes by microarray analysis for performing expression QTL analysis (eQTL). By the results of trait QTL, we identified 83 QTLs involved in determining the panicle structure. Farther, we suggested a validity of comparative analyses between trait-QTL and eQTL data for prediction of candidate genes of QTL. We first focused on OsCKX2/Gn1a, which is involved in increasing the secondary rachis number per panicle and already isolated by a positional cloning previously, and succeeded to predict the most feasible candidate of the Gn1a locus. Using the same method, we succeeded to predict several candidates for qPBL-6, qPBL-7 and pUPB-8.