Biophysical characterization of protein interactions within a transcription factor network

基因表达部分地通过称为转录因子和辅助调节因子的蛋白质对和蛋白质组之间的相互作用来调节。这些蛋白质在基因启动子和增强子处组装成复合物，从而控制该基因的表达。在分子水平上对这些复合物如何形成以及不同的相互作用如何相互合作或竞争知之甚少。在这个提议中，我们的目标是定义一个复杂的两个转录调节因子（HOP和SRF）参与心脏发育，并开始定义其他的相互作用，使一个转录网络的发展一个正常的心脏必不可少的。

Gene expression is regulated in part by interactions between pairs and groups of proteins known as transcription factors and co-regulators. These proteins assemble into complexes at gene promoters and enhancers and thereby control the expression of that gene. Little is known at the molecular level of how these complexes form and how different interactions cooperate or compete with each other. In this proposal we aim to define a complex between two transcriptional regulators (HOP and SRF) involved in cardiac development and to begin to define other interactions that make up a transcriptional network essential for development of a normal heart.