A comprehensive analysis of the outer membrane, surface exposed and secreted proteome of Pasteurella multocida

多杀性巴氏杆菌是一系列动物疾病的病原体。多杀性巴氏杆菌致病的分子机制知之甚少，目前的疫苗通常无效。我们将通过全局蛋白质组学方法鉴定在自然感染期间或在模拟自然感染的条件下表达的所有多杀性巴氏杆菌外膜、表面暴露和分泌蛋白。我们相信分泌的蛋白质和那些在细菌细胞外表面发现的蛋白质可能是关键的毒力决定因素。预期的结果是确定了一些候选疫苗抗原，并加强了对巴氏杆菌发病机制的了解。

Pasteurella multocida is the causative agent of a range of animal diseases. The molecular mechanisms of P. multocida pathogenesis are poorly understood and the current vaccines generally ineffective. We will identify all P. multocida outer membrane, surface exposed and secreted proteins expressed during natural infection, or under conditions which mimic natural infection, by a global proteomics approach. We believe that secreted proteins and those found on the outer surface of the bacterial cell are likely to be crucial virulence determinants. The expected outcomes are the identification of a number of candidate vaccine antigens and an enhanced understanding of Pasteurella pathogenesis.