4-laser cell sorter

在FCiT中，几个不同类别的生命科学和生物医学导向的科学设备将汇集在一起，共同运作。这些设备特别服务于基础研究和临床前研究之间的接口。4激光细胞分选器是FCiT（定量蛋白质组学，细胞和表型筛选，基因组学和单细胞，人体3D（芯片）组织）中涉及的几个技术平台的补充，并支持Riedberg校区的个人项目。细胞分选器的范围包括用于CRISPR/Cas筛选，组织细胞分选，亚群富集（例如，在稳定细胞系生成期间）和细胞表征（FACS分析）。仪器配备4个激光器（405nm、488nm、561nm、638nm）和6个荧光探测器。激光共线，可以分配到任何6个荧光pmt。所安装的滤光片集允许检测标准荧光团和6个荧光和两个散射参数的整体同时检测。根据检测参数，该设备允许双向分选，包括在微孔板（6孔、12孔、24孔、48孔、96孔和384孔）中进行单细胞分选和索引分选。与其他液滴分选器相比，该设备具有微流控芯片设计（分选芯片尺寸为70 μ m， 100 μ m和170 μ m），并且可以轻松地由用户交换微流控分选芯片以及整个样管。这样可以最大限度地减少可能的污染，并允许多用户操作以及在同一仪器上对不同大小的细胞和生物体（例如细菌，酵母和哺乳动物细胞）进行最佳分选。

In the FCiT, several life science and biomedical oriented scientific devices in different categories will be brought together and jointly operated. The devices serve in particular the interface between basic research and pre-clinical research. The 4-laser cell sorter is for the complementation of several technology platforms involved in the FCiT (Quantitative proteomics, Cellular & phenotypical screening, Genomics & single cell, Human 3D (chip) tissues) and for the support of individual projects at the Riedberg Campus. The scope of the cell sorter includes use in CRISPR/Cas screens, tissue cell sorting, subpopulation enrichment (e.g., during stable cell line generation), and cell characterization (FACS analyses). The instrument is equipped with 4 lasers (405nm, 488nm, 561nm, 638nm) and 6 fluorescence detectors. The lasers are collinear and can be assigned to any of the 6 fluorescence PMTs. The installed filter set allows detection of standard fluorophores and overall simultaneous detection of 6 fluorescence and two scatter parameters. Based on the detected parameters, the device allows 2-way sorting, including single-cell sorting in microplates (6-, 12-, 24-, 48-, 96- and 384-well) with index sorting. As a special feature compared to other droplet sorters, the device has a microfluidic chip design (sorting chips available in 70µm, 100µm and 170µm sizes) and the possibility to easily exchange both the microfluidic sorting chips as well as the entire sample tubing by the user. This minimizes possible contamination and allows both multiuser operation as well as optimal sorting of cells of different sizes and organisms on the same instrument (e.g. bacteria, yeasts and mammalian cells).