Molecular and cell biological study on the expression of germ cell function.

[顶体物质的运输]当在培养基中加入囊泡运输抑制剂breferdin时，顶体的形成受到阻碍(Tanji et al. (1998) J Electron Microsc, 47；161)。[附睾成熟过程中acrin 1的修饰（MN7=90K）]随着附睾成熟过程中形态的改变，acrin 1改变了其定位（Yoshinaga et at.）。（1998年）第292单元；427年)。[赤道蛋白的作用（MN9=48K）]赤道蛋白在精子到达卵泡周围间隙之前一直停留在赤道段，尽管顶体反应过程中释放了一定量的赤道蛋白。在体外受精（IVF）条件下，在抗赤道蛋白抗体（mMN9）存在的情况下，精子不能进入卵子，停留在卵泡周围空间。皮质颗粒不能从卵中释放出来。原核形成和2细胞形成均受到明显抑制。因此，赤道蛋白被认为是精子-卵子膜融合的必要分子。(Toshimori et al. (1998) Biol repd, 59；22)。[acrin 1和acrin 2的作用（MC41=165K）]顶体反应需要acrin 1，顶体反应后穿透透明带之前需要acrin 2。（Saxena et al. (1999) J repd Fert, in press。）【acrin 3的功能（mc101 =155K）】acrin 3被认为与配子融合有关，但其具体机制尚不清楚。【MC31抗原的克隆（26-35K）】MC31与大鼠精子的CE9分子高度同源，是免疫球蛋白超家族分子。【综述】对精子抗原分子的成熟进行了综述。(Toshimori K（1998）细胞组织研究，293；177年)。

[Transport of acrosome materials] The fonnation of the acrosome was hamperd when the vesicle transport inhibitor, breferdin, was added to the culture medium (Tanji et al. (1998) J Electron Microsc, 47 ; 161). [Modification of acrin 1 (MN7=90K) during epididymal maturation] Acrin 1 changed its localization accompanying the morphological change during epididymal maturation (Yoshinaga et at. (1998) Cell Tiss Res, 292 ; 427.). [Function of equatorin (MN9=48K)] The equatorin remained at the equatorial segment until sperm reached to the perivitelline space, although some amount of the equatorin was released during the acrosome reaction. Sperm could not penetrate into the egg, and remained in the perivitelline space under in vitro fertilization (IVF) condition in the presence of anti-equatorin antibody (mMN9). The cortical granules could not be released from the egg. Both pronuclear formation and 2-cell formation were inhibited significantly. Thus, the equatorin is found to be an essential molecule for sperm-egg membrane fusion. (Toshimori et al. (1998) Biol Reprod, 59 ; 22.). [Function of acrin 1 and acrin 2 (MC41=165K)] Acrin 1 was required for the acrosome reaction, while acrin 2 was required for a certain process during after acrosome reaction but before penetration through the zona pellucida. (Saxena et al. (1999) J Reprod Fert, in press.) (Function of acrin 3 (MC1O1=155K)] Acrin 3 was suggested to be related to the gamete fusion, but the detail of the mechanism remained unclear. [Cloning of MC31 antigen (26-35K)] MC31 was highly homologous to the CE9 molecule of rat sperm, which is a molecule of immunoglobulin superfamily. [Review] The maturation of sperm antigenic molecules was reviewed. (Toshimori K (1998) Cell Tissue Res, 293 ; 177.).