Prostaglandin production in the plerocercoid of Spirometra erinaceieuropaei and its function in larva migrans

研究了温度、pH值和寄主脂肪酸对粉纹夜蛾脂肪酸含量的影响。与37ﾟC孵育相比，18ﾟC孵育24小时后，磷脂部分的omega-6系列脂肪酸，尤其是花生四烯酸增加。甘油三酯部分多不饱和/饱和脂肪酸的比例变化与生理盐水孵育6小时的磷脂部分相反。在37ﾟC时，游离脂肪酸组分中的花生四烯酸在前3小时下降，在10ﾟC时增加，而游离脂肪酸组分中的花生四烯酸在前1小时下降。并持续增加至3小时后再次下降。研究了5 mM花生四烯酸作用0、0.5、1、3、6h后青海海鞘磷脂和甘油三酯组分的脂肪酸组成变化。在37ﾟC，花生四烯酸在甘油三酯组分中被迅速吸收并结合，2-3小时后下降，但在10ﾟC时生长缓慢。在剂量依赖过程中，0.5、1、2和5 mM花生四烯酸孵育的文昌鱼中可检测到PGE2，2和5 mM花生四烯酸可检测到PGE2。前列腺素D_2和前列腺素E_2总量随花生四烯酸浓度的增加而增加，未检测到PGF_2α和6-酮-PGF_1α。在时间依赖性过程中，先在15分钟检测到PGF_1α，然后在30分钟检测到6-酮-PGF_1α，在1小时检测到前列腺素D_2和前列腺素E_2。血栓素B_2在剂量依赖和时间依赖过程中均未检测到，在5 mM花生四烯酸孵育1小时时仅检测到PGE_2。

The effects of temperature, pH, and host fatty acids on the fatty acid contents of Spirometra erinaceieuropaei plerocercoids were investigated. After 24 hr incubation at 18ﾟC in host snake serum, omega 6 series fatty acids, especially arachidonic acid, in the phospholipid fraction of the plerocercoids, increased compared with those incubated at 37ﾟC.The change in the ratio of polyunsaturated to saturated fatty acids in the triglyceride fraction was opposite to that observed for the phospholipid fraction after incubation in physiological saline for 6 hr. At 37ﾟC, arachidonic acid in the free fatty acid fraction decreased for the first 3 hr, and increased, at 10ﾟC, however, arachidonic acid in the free fatty acid fraction decreased for the first 1 hr. and increased until 3 hr, and decreased again. The changes of the fatty acid composition of phospholipid and triglyceride fractions in Spirometra eninaceieuropaei plerocercoid were investigated after 0, 0.5, 1, 3, 6 hr with 5 mM arachidonic acid. At 37ﾟC, arachidonic acid was absorbed and incorporated rapidly in triglyceride fraction and decreased after 2-3 hr, but growth slowly at 10ﾟC.In the dose-dependent course, PGD_2 was detected in the plerocercoids incubated with 0.5, 1, 2 and 5 mM arachidonic acid and PGE_2 was detected with 2 and 5 mM arachidonic acid. Total amounts of PGD_2 and PGE_2 increased with increased concentrations of arachidonic acid, and PGF_<2 alpha> and 6-keto-PGF_1 alpha were not detected. In the time-dependent course, PGF_1 alpha was first detected at 15 min, thereafter, 6-keto-PGF_1 alpha a was detected at 30 min and PGD_2 and PGE_2 were detected at 1 hr. Thromboxane B_2 was not detected in either the dose-dependent or time-dependent course, and only PGE_2 were detected in the incubation medium with 5mM arachidonic acid at 1 hr.