Replication and division of the structure in plant cells

在多种植物细胞中，对细胞核、线粒体、双胞体、鞭毛和细胞壁微管排列等细胞器的复制和分裂进行了协同研究。1)利用电镜研究了野生型和腺苷酸环化酶缺陷型酵母SPB的重复，并用冷冻取代法发现了一种特殊的s形纺锤体。2)研究了芽殖酵母和绒泡菌疟原虫中线粒体类核的融合和分裂，制备了结合线粒体DNA的膜组分。3)细胞松弛素B和western blotting检测结果表明，花青菜叶绿体分裂面周围有一个由肌动蛋白丝组成的环状结构。在不同的生理条件下，研究了细叶茅细胞周期中细胞核与叶绿体的密切联系。4)利用电镜研究了叶氏梭菌合子萌发过程中dictysomal的生长和分裂情况，以及尖苞藓dictysomal的结构和功能。5)应用免疫荧光显微镜研究了丝绵草和长春花细胞壁微管的排列方式。6)研究了植物激素（生长素、赤霉素、细胞分裂素）对百日草叶肉细胞皮层微管排列和DNA合成的影响。采用极化辐照和细胞离心的方法分析了蓝蕨原丝细胞内蓝光诱导细胞分裂的光反应位点。7)研究了鞭毛器的复制与有丝分裂纺锤体发育的关系。

Researches on the replication and division of cellular organelles such as nuclei, mitochondria, dictyosomes, flagella and the cell wall microtubule arrangements were cooperatively performed in a variety of plant cells. 1) Duplication of SPB of both wild and adenylate cyclase-deficient mutant of the yeast Saccharomyces cerevisiae was studied by electron microscopy, and a peculiar sigmoidal spindle was also found by freeze substitution method. 2) Fusion and division of the mitochondrial nucleoid were studied in the budding yeast and in plasmodia of Physarum, and a membrane fraction binding the mitochondrial DNA was prepared. 3) Effects of cytochalasin B and western blotting showed that a ring structure around the dividing plane of Cyanidium caldarium chloroplast consisted of actin filaments. Close association between the nucleus and chloroplasts in the cell cycle of Euglena gracilis was studied under different condition physiological conditions. 4) Growth and division of the dictyosomes during zygote germination of Closterium ehrenbergii and the structure and function of dictyosomes in Scenedesmus acuminatus were studied by electron microscopy. 5) The arrangements of wall microtubules in Spirogyra and in Vinca major were studied by immunofluorescent microscopy. 6) Effects of plant hormones (auxin, gibberellin, cytokinin) were studied in relation to the arrangement of cortical microtubules and DNA synthesis in isolated Zinnia mesophyll cells. Intracellular photoreactive site for blue light-induced cell division in protonemata of the fern was analyzed by polarized irradiation and cell centrifugation. 7) Replication of the flagellar apparatus in the alga Prasinophyceae was studied in relation to the development of mitotic spindle.