A research on shared antigen between myelin-associated glycoprotein and lymphocytes

髓鞘相关糖蛋白是髓鞘的一种次要成分，与人类淋巴细胞，特别是自然杀伤(NK)细胞有共同的抗原。我们在这一研究领域完成了以下工作：1.我们已经制备了19种抗MAG的单抗，并对其特性进行了检测。鉴定了3组抗体：识别MAG分子多肽部分的免疫球蛋白抗体和识别MAG分子糖基的免疫球蛋白抗体和免疫球蛋白M抗体。与抗Leu-7相关的有2组，其中1组与抗Leu-7.2具有相似的抗原特异性，另一组与抗Leu-7.2有轻微的抗原特异性。用抗MAG单抗检测HSB-2细胞(抗Leu-7免疫原)和外周血淋巴细胞的抗原蛋白分子。免疫印迹上识别了几种蛋白质分子。识别MAG碳水化合物部分的抗MAG单抗在体外抑制NK活性。建立了检测抗MAG抗体的ELISA法，发现部分多发性硬化症和格林-巴利综合征患者抗MAG滴度升高。建立了用2种单抗进行MAG定量的酶联免疫吸附试验体系。我们用抗MAG的单抗通过免疫组织化学方法显示MAG与肺癌之间存在交叉抗原性。

Myelin-associated glycoprotein is a minor constituent of myelin and has a shared antigen with human lymphocytes especially with natural killer (NK) cells. We have completed followings on this research field.1. We have generated 19 kinds of anti-MAG monoclonal antibodies and examined those characters. There groups of antibodies were indentified: IgG antibodies recognizing the polypeptide moiety and IgG and IgM antibodies recognizing the carbohydrate moiety of MAG molecule. Relating with anti-Leu-7 2 groups were identified, one has similar and the other has slightly defferent antigenic specificity with anti-Leu-7.2. Antigenic protein molecules in HSB-2 cells (immunogen of anti-Leu-7) and lymphocytes in peripheral blood were examined using monoclonal anti-MAG antibodies. Several protein molecules were recognized on immunoblots.3. An anti-MAG monoclonal antibody which recognized carbohydrate part of MAG inhibited NK activity in vitro.4. We developed ELISA system for detection of anti-MAG antibody and found some patients with multiple sclerosis and Guillain-Barre syndrome have elevated anti-MAG titer.5. We also developed ELISA system for quantitation of MAG using 2 kinds of monoclonal antibodies.6. We showed a cross antigenicity between MAG and lung cancer using a monoclonal anti-MAG antibody immunohistochemically.