Protective immunity against Brugia malayi by monoclonal antibodies

用BALB/c小鼠检测了20多种抗马来丝虫单克隆抗体对感染性和早期L4幼虫的保护能力。在用100 L3的B攻击感染之前立即腹膜内注射单克隆抗体。马来语。然而，没有一个显示蠕虫恢复显著减少。然后，用亲和纯化的微丝蚴单克隆抗体免疫小鼠，并在攻击感染后两周检查虫负荷。在用IgM单克隆抗体（Ov H）纯化的抗原免疫的小鼠中，观察到超过70%的蠕虫回收率降低。在用这些分级抗原免疫的小鼠中测量保护性免疫。在用33-35或40-45 KDa抗原免疫的小鼠中观察到超过90%的蠕虫回收减少。结果表明，小鼠模型可作为评价抗马氏丝虫攻击感染保护力的有效工具，也可作为疫苗研制中纯化保护性抗原的可靠和省时的模型。

More than 20 monoclonal antibodies generated against human filarial parasite, Brugia malayi, were assayed for the ability to protect against infective and early L4 larvae in BALB/c mice. The monoclonal antibodies were injected intraperitoneally immediately before challenge infection with 100 L3 of B. malayi. However, non of them revealed significant reduction in worm recovery. Then, mice were immunized with affinity purified antigens from microfilariae using monoclonal antibodies, and were examined worm burden two weeks after challenge infection. In mice immunized with antigens purified with an IgM monoclonal antibody (Ov H), more than 70% reduction in worm recovery were observed.Microfilarial antigens were fractionated according to molecular weights using SDS-PAGE. Protective immunity was measured in mice immunized with these fractionated antigens. More than 90% reduction in worm recovery were observed in mice immunized with 33-35 or 40-45 KDa antigens. On the other hand, significant reduction was not shown in mice immunized with 60-170 KDa antigens.The results indicate that murine model may be a useful tool to evaluate the protection against challenge infection of Brugia malyi and be a reliable and time-saving model to purify the protective antigens for vaccine development.