Study on colonization factors of V. cholerae non-01

研究了非01霍乱弧菌定植的两个可能因素：1)菌毛：一些非01霍乱弧菌在CFA琼脂上培养时发现产生以前未描述的菌毛。菌毛形态与本研究中发现的副溶血性弧菌菌毛形态相似。Non-01霍乱弧菌菌毛的抗原性不仅不同于副溶血性弧菌菌毛，而且与产肠毒素大肠杆菌菌毛的抗原性也不同。2)蛋白酶：证实非01霍乱弧菌能产生蛋白酶。该酶被纯化为均一，并与霍乱弧菌01的一种已知的定植因子进行了比较。与霍乱弧菌01的蛋白水解酶一样，Non-01霍乱弧菌产生的水解酶是一种兼具蛋白酶活性和血凝活性的双功能蛋白。理化和免疫学分析表明，这两种酶是相同的。

Following two factors possibly involved in colonization of V.cholerae non-01 were studied.1) Pili: Some strains of V.cholerae non-01 were found to produce previously undescribed pili when they were cultured on CFA agar. The pili morphology was similar to that produced by v.parahaemolyticus, which was also found in this study. Antigenicity of the pili of V.cholerae non-01 was different from not only pili of V.parahaemolyticus, but also various colonization factors (pili) of enterotoxigenic Escherichia coli.2) Protease: Production of protease by V.cholerae non-01 was demonstrated. The protease was purified to homogeneity, and compared with a protease known as a colonization factor of V.cholerae 01. Like the protease of V.cholerae 01, the protease produced by V.cholerae non-01 was demonstrated to be bifuntional proteins possessing both activities of protease and hemagglutination. Physicochemical and immunological analysis suggested that the both proteases were identical.