The experimental chemotherapy on human salivary gland cancer.

人涎腺腺癌细胞系（HSG）在仅存在二丁酰环腺苷酸（dB-cAMP）、顺二氨二氯铂（CDDP）或丝裂霉素C（MMC）的情况下培养，或在dB-cAMP和每种顺铂药物的组合的情况下培养。然后检测经处理的HSG细胞的姐妹染色单体交换（SCE）诱导、半固体琼脂培养基中的集落形成效率（CFE）、细胞ras癌基因p21水平和活细胞计数测量的细胞存活率。已经发现，当与仅用CDDP或MMC处理的细胞相比时，在dB-cAMP存在下用CDDP或MMC处理细胞显著增加SCE诱导的频率。用顺铂或丝裂霉素C单独或联合用药处理裸鼠移植瘤细胞株，观察其CFE、rasp 21水平和细胞存活率的变化。化疗药物联合dB-cAMP化疗对肿瘤生长的抑制作用明显优于单纯化疗药物。此外，dB-cAMP处理的肿瘤巢显示Miosin和S-100蛋白链的免疫组化染色阳性，这些结果表明dB-cAMP处理的人涎腺腺癌在裸鼠体内的肿瘤生长、SCE诱导、CFE和CDDP或MMC暴露的细胞表面的改变。

A human salivary gland adenocarcinoma cell line(HSG) was cultivated in the presence of dibutyryl cyclic AMP(dB-cAMP), cis-diamminedichloroplatinum(CDDP) or mitomycin C(MMC) only, or of the combination of dB-cAMP and each of the antineoplastic drugs. Then the treated HSG cells were examined for the inductio of sister chromatid exchanges(SCEs), colony-forming efficiency(CFE) in semisolid agar medium, cellular ras oncogen p21 level and cell survival as measured by counting for viable cells. It has been found that the frequency of SCEs induction is significantly increased by treatment of cells with CDDP or MMC under the presence of dB-cAMP, when compared to that in the cells treated with CDDP or MMC only. Moreover, marked reduction in CFE, ras p21 level and cell survival were found to occur inthe cells treated with the combination of dB-cAMP and CDDP or MMC.The human adenocarcinoma produced by transplantation into nude mice of HSG cells was treated with CDDP or MMC only or combination of dB-cAMP and these antineoplastic drugs. The antineoplastic drugs plus dB-cAMP chemotherapy resulted in more marked suppression of tumor growth than antineoplastic drugs only therapy. In addition, dB-cAMP treated tumor nest showed immunohistrochemical positive staining for miosin and s-100 protein -chain.These findings indicate that treatment of a human salivary gland adenocarcinoma with dB-cAMP results in modification of tumor growth in athymic nude mice, SCE induction, CFE and cell survical in the cells exposed to CDDP or MMC.