Construction of the Rat Chromosomal Map by DNA Probes.

1.通过DNA探针、回交分析和原位杂交对大鼠基因进行染色体定位。(1)血管紧张素原（Angiotensinogen，Ang）：应用cDNA和Southern杂交技术，在近交系大鼠中检测到3种Ang等位基因。两组回交分析结果表明，Ang与其它16个位点均不连锁，原位杂交结果表明，Ang与19 q区域连锁。(2)胰岛素-1（Ins-1）：荧光原位杂交（FISH）将Ins-1定位于1 q55，Southern印迹分析检测到该基因座的4个等位基因。Ins-1与位于1号染色体上的c、Hbb等其他22个基因座没有连锁。这种差异可能是由于它们在染色体上的距离遥远。(3)Renin（Ren）：通过FISH将Ren指定为13 ql。由于Ren基因第一内含子存在VNTR，在11个近交系大鼠中共检测到5个等位基因。回交分析表明Ren与Fh（20 cM）和Pep-3（13 cM）连锁。在13号染色体上的排列顺序为Fh-Ren- Pep-3. (4)β_2-微球蛋白（B_2m）：Southern杂交检测到3个B_2m位点的RFLP，回交分析表明B_2m位点与zi（2或9 cM）、Hao-1（6cM）和Srp-1（29 cm）连锁。通过小鼠和大鼠细胞制备的18个体细胞杂交克隆，将B2 m定位于3号染色体。利用体细胞杂种克隆进行生化基因的染色体定位。我们在小鼠和大鼠细胞之间制备了一组18个体细胞杂交克隆。这一面板使我们能够新分配21个生化基因的大鼠染色体1，2，3，4，7，8，9，10，12，13，14，19和20，分别。进一步证实Acp-1和Glo-1分别位于第6和20号染色体上。

1. Chromosomal assignment of the rat genes by DNA probes, backcross analysis, and in situ hybridization. (1) Angiotensinogen (Ang) : 3 alleles of Ang were detected among inbred strains of rats by using the cDNA and Southern blot analysis. Ang was not linked with other 16 loci using 2 sets of backcross analysis, but was assigned to 19q region by in situ hybridization. (2) Insulin-1 (Ins-1) : Ins-1 was assigned to 1q55 by fluorescence in situ hybridization (FISH) , and 4 alleles of the locus were detected by Southern blot analysis. Ins-1 was not inked with other 22 loci including c, Hbb which located on chromosome 1. This discrepancy may be due to the remote distance between them on the chromosome. (3) Renin (Ren) : Ren was assigned to 13ql by FISH. Since Ren gene have a VNTR within the 1st intoron of the gene, 5 alleles were detected among 11 inbred strains of rats. Backcross analysis revealed the linkages of Ren with Fh (20cM) and Pep-3 (13cM). The order on chromosome 13 was determined as Fh - Ren - Pep-3. (4) beta2-microglobulin (B2m) : 3 RFLPs by using B2m probe were detected by Southern blot analysis, and backcross analysis revealed the linkage of B2m locus with zi (2 or 9cM), Hao-1 (6cM) , and Srp-1 (29cm) . B2m was assigned to chromosome 3 by a panel of 18 somatic cell hybrid clones made by mouse and rat cells.2. Chromosomal assignment of biochemical genes by somatic cell hybrid clones. We made a panel of 18 somatic cell hybrid clones between mouse and rat cells. This panel enabled us to newly assign 21 biochemical genes of rats on chromosome 1, 2, 3, 4, 7, 8, 9, 10, 12, 13, 14, 19, and 20, respectively. Further Acp-1 and Glo-1 were reconfirmed to locate on chromosome 6 and 20, respectively.