Immuno-pathological studies in IgA nephropathy : The role of virus for etiological and progressive factors

为了建立一种实验性的IgA肾病模型，对接种病毒的小鼠进行了组织学和病毒学研究。首先，用纯化的柯萨奇B4病毒对小鼠进行两次静脉接种，间隔1-28天。在第二次接种36小时后，从每只小鼠身上获取肾脏，并检查组织学变化，如系膜沉积。在间隔14天或更长时间的两次接种中，小鼠的系膜沉积明显，这表明在足够长的间隔内重复抗原刺激是产生系膜沉积所必需的。另一组4周龄的小鼠每隔4周接种4-8次柯萨奇B4病毒(静脉注射)。组)或腹膜腔内注射(ip.组)。每只小鼠在最后一次接种后1个月取肾脏，进行组织学检查。在静脉注射中。光镜显示4…后肾小球系膜内有PAS阳性沉积更多的疫苗接种。接种6次或6次以上后沉积更明显，并伴有不均匀的环壁肥大和新月体的形成。免疫荧光检测，免疫后4~5次免疫标本中，免疫球蛋白G染色最深，免疫后6次或6次以上，部分标本中免疫球蛋白A和免疫球蛋白免疫球蛋白染色程度相同。所有标本均为病毒抗原阳性，染色强度随接种次数的增加而增加。电子显微镜下可见上皮下和内皮下军团内有沉积。在IP中。光镜下可见接种4次或4次以上的肾小球系膜内有PAS阳性沉积。接种6次或以上后，基底膜不均匀肥大，但无新月体形成。免疫荧光显示，与对照组相比，肾小球系膜中的免疫球蛋白、免疫球蛋白A和免疫球蛋白M染色较深，且以免疫球蛋白A为主。所有标本病毒抗原均为阳性。电子显微镜检查结果与静脉注射的结果相似。一群人。由此证明，间歇性接种柯萨奇B4病毒对小鼠的肾脏损害与人类IgA肾病非常相似。较少

In order to produce an experimental model of IgA nephropathy, a histologiral and virological study of mice inoculated with a virus was carried out. First, mice were intravenously inoculated twice at an interval of 1-28 days purified with Cox.B4 virus. The kidneys were obtained from each mouse 36 hours after the second inoculation, and examined for histological changes such as mesangial deposits. Mesangial deposits were marked in mice given two inoculation at an interval of 14 days or more, indicating that antigen stimulations repeated at a sufficiently long interval are necessary for producing mesangial deposits. Another series of 4-week-old mice were inoculated with Cox.B4 virus 4-8 times at 4-week intervals either intravenously (i.v. group) or intraperitoneally (i.p. group). The kidneys were obtained from each mice one month after the final inoculation, and examined for histological changes. In the i.v. group, light microscopy revealed PAS- positive deposits in the mesangium after 4 … More inoculations. The deposits were more marked after 6 or more inoculations, accompanied by uneven hypertrophy of the loop wall and formation of crescents. By immunofluorescence, IgG was most deeply stained in speciments obtained after 4 or 5 inoculations, whereas IgA and IgG were stained to the same degree in some speciments after 6 or more inoculations. All speciments were positive for virus-Ag, the intensity of staining being increased in proportion to the number of inoculations. Electron microscopy disclosed deposits in subepithelial and subendothelial legions. In the i.p. group, light microscopy showed PAS-positive deposits in the mesangium after 4 or more inoculations. After 6 or more inoculations, there was uneven hypertrophy of the basement membrane, but no formation of crescents. By immunofluorescence, IgG, IgA and IgM were deeply stained in the mesangium in comparison with controls; IgA was always stained predominantly. Virus-Ag was positive in all speciments. Electron microscopic findings were similar to those in the i.v. group. It was thus demonstrated that intermittent intraperitoneal inoculation with Cox.B4 virus in mice produces renal lesions closely resembling those in human IgA nephropathy. Less