Immunohistochemical Study on the Occurrence, Morphology and Development of the Gastrointestinal Brush Cells of the Rat

刷状细胞，也称为簇状细胞，是已知存在于许多哺乳动物物种的内胚层来源的上皮中的罕见细胞类型。在本研究中，我们发现一种抗肝型脂肪酸结合蛋白（L-FABP）的抗体特异性免疫染色大鼠胃肠刷状细胞，并利用这种免疫反应性作为刷状细胞的特异性标记来阐明其以前未阐明的方面。在胃中，刷状细胞在贲门区的沟内成群存在，在胃底和幽门区的表面和小凹上皮中散在存在单个刷状细胞，L-FABP免疫反应强烈。在形态学上，明确了所有刷状细胞都有一个薄的基底细胞质突起，没有分泌颗粒。在大鼠的个体发育过程中，新生大鼠胃刷状细胞首先出现，L-FABP免疫反应阳性，随着年龄的增长，刷状细胞逐渐增多， ...更多信息 艾德出生后2周内，胆管上皮内出现刷状细胞。在8 ~ 16周时，胆管上皮细胞数量显著增加，占总胆管上皮细胞的30%左右。雌性刷状细胞的初始发育比雄性延迟约2周。另一方面，L-FABP免疫反应刷状细胞仅在产后8周后首次出现。16周后刷状细胞数量逐渐增多，但40周时仅占刷状细胞总数的25%左右，本研究利用特异性组织化学标记，对刷状细胞的分布、形态和个体发生提供了新的发现。此外，L-FABP在刷状细胞中的表达可能揭示这种细胞类型与脂肪酸代谢有关的功能方面。少

The brush cell, also referred to as the tuft cell, is a rare cell type known to occur in the endoderm-derived epithelia of many mammalian species. In the present study, we found that an antibody against Liver-type Fatty Acid-Binding Protein (L-FABP) specifically immunostained the gastrointestinal brush cells of the rat, and utilized this immunoreactivity as a specific marker of the brush cell to elucidate its previously unclarified aspects.The largest populations of brush cells were found in the stomach and common bile duct. In the stomach, brush cells were present in a group in the groove of cardiac region as well as scattered singly in the surface and foveolar epithelia of the fundic and pyloric regions, with intense immunoreactivity for L-FABP. Morphologically, it was clarified that all brush cells had a thin basal cytoplasmic process without secretory granules. In the ontogeny of rats, brush cells first occurred in the new born stomach, with immunoreactivity for L-FABP, and increas … More ed remarkably in number within the few weeks following the end of the first 2 weeks of sackling period.In the common bile duct, The brush cells as recognized by scanning electron microscopy first appeared in the epithelium at 4 weeks after birth. They showed a remarkable increase in number between 8 and 16 weeks and finally occupied about 30 % of the total epithelial cell population of common bile duct. The initial development of brush cells in the females delayed for approximately 2 weeks from that of males. On the other hand, the L-FABP-immunoreactive brush cells first appeared only after 8 weeks postpartum. They showed a gradual increase in number after 16 weeks but reached only about 25 % of the total brush cell population by the time of 40 weeks.The present study, by virtue of the specific histochemical marker, provided new findings concerning the distribution, morphology and ontogeny of the brush cell. Also, the expression of L-FABP in the brush cell may shed light on the functional aspect of this cell type in relation to the fatty acid meTabolism. Less