Basic Research on Biological Monitoring of Industrial Chemicals in Japan

1.建立了以对离子为流动相的高效液相色谱同时测定肌酐和氯苯代谢物4-氯邻苯二酚和对氯苯酚的方法。接触结束前1、2、3、4h尿中有机溶剂(甲苯、二甲苯、氯苯、三氯乙烯)的尿代谢物浓度由高到低依次为：末次排尿至接触结束的时间间隔对尿液代谢产物浓度的测定起着重要的控制作用。为了储存尿液代谢物，将一张滤纸浸泡在接触甲苯的工人的尿液中，并将其晾干。将尿液中的马尿酸和肌酐溶于50%甲醇中，用高效液相色谱法测定其浓度。甲醇提取物和原尿中马尿酸/肌酐浓度的比值相近。为了评价测定尿液中代谢物浓度的校正方法，测定了空气中甲苯浓度与尿中马尿酸实际浓度和校正浓度之间的相关系数。空气中甲苯浓度与尿中马尿酸浓度之间的相关系数，经肌酐校正后高于按比重或实际浓度校正后的数值。

1. Simultaneous determination of creatinine and chlorobenzene metabolites, 4-chlorocatechol and p-chlorophenol, by High Performance Liquid Chromatography (HPLC) using paired ion as a mobile phase was conducted.2. Concentrations of urinary metabolites of organic solvents (toluene, mxylene, chlorobenzene and trichloroethylene) in 1, 2, 3 and 4 hours before the end of exposure were in the descending order, indicating that the interval between the last urination and the end time of exposure plays an importantrole for determining urinary concentration of the urinary metabolites.3. For a purpose of storage of urinary metabolites, a piece of filter paper was dipped in urine of workers exposed to toluene and dried. Urinary hippuric acid and creatinine in the paper were dissolved into 50% methanol and their concentrations were determined by HPLC. The ratio of hippuric acid to creatinine concentration in methanol extract and that in original urine was similar to each other.4. In an attempt to evaluate the correction methods for determination of metabolite concentration in urine, the correlation coefficient between the toluene concentration in the air and the actual or corrected concentration of hippuric acid in the urine was determined. The correlation coefficients between the toluene in the air and the hippuric acid concentration in the urine corrected for creatinine was higher than that corrected for specific gravity or actual concentration.