Regional Specificity of the Mode of Transmission of Bovine Leukemia Virus and of Garcinogenecity of Viral Tumors

在1991 - 1992年的两年时间里，我们与中国长春兽医学院进行了牛白血病的国际联合研究。第一年：对370头牛（中国产黄牛257头，荷斯坦牛113头）进行血液学和牛白血病病毒（BLV）抗体调查。采用琼脂凝胶免疫扩散反应（AGID），用BLV诊断抗原（北中抗原）进行抗体检测。结果表明，BLV抗体在所有奶牛中均为阴性，但在1头肉牛中呈阳性。血液学检查发现3头肉牛和15头奶牛出现淋巴细胞增多症（符合Bendixbnls白血病诊断标准）。所有病例均为血原虫阴性。第二年：采用AGID方法对荷斯坦牧场180头猪进行BLV抗体检测。对长春兽医学院保存的牛白血病标本进行了组织学、免疫组化和电镜检查。结果，所有奶牛BLV抗体均为阴性，表明上述牧场未受到BLV污染，这可归因于引入新奶牛时采用AGID方法进行的BLV抗体检测成功。目前在中国发生的牛白血病也经常是B淋巴细胞起源，与日本发生的流行（成人型）白血病相似。

During a two-year period 1991 - 1992, we made an international joint study on bovine leukemia with Changchun Veterinary College in China.For the 1st year : Hematological and bovine leukemia virus (BLV) antibody investigation were made on a total of 370 heads [257 of yellow cow (beef cattle) of Chinese origin and 113 of Holstein (dairy cattle)]. The antibody investigation was made on the basis of agar gel immunodiffusion reaction (AGID) using BLV diabnostic antigen (Kitasato antigen). As a result, BLV antibody proved to be negative in all dairy cattle, but positive in one of the beef cattle. Hematological investigation revealed lymphocytosis (according to the Bendixbnls leukemia diagnostic criteria) in 3 beef and 15 dairy cattle. All cases werd blood protozoa-negative.For the 2nd year : BLV antibody investigation using the AGID method was made on 180 heads of a Holstein pasture. Histological, immunohistochemical and electron microscopic examinations were also made of those bovine leukemic materials which had been preserved by Changchun Veterinary College. As a result, all dairy cattle proved to be BLV antibody-negative, indicating non-BLV contamination of the above pasture, and this was considered to be ascribable to that successful BLV antibody examination by AGID method which has been made upon introduction of new cows. Bovine leukemia now occurring in China was also revealed to be frequently of B lymphocytic origin and similar to epidemic (adult type) leukemia occurring in Japan.