Analysis of expression of tumor-associated antigen and its clinical application in oral cancers.

应用荧光原位杂交技术可检测到口腔肿瘤中17号染色体数目的畸变，并与口腔肿瘤的恶性行为相关。PCNA免疫反应与口腔鳞状细胞癌的分化程度和分化程度有关。PCNA评分在癌前病变和癌前病变中明显增高，且与癌前病变的异型增生程度相关。鳞癌化疗后PCNA评分明显下降。根据这一结果，癌细胞对抗癌剂的反应可以通过连续测量PCNA来估计。c-erbB-2癌蛋白在涎腺恶性肿瘤中很少表达。然而，由于c-erbB-2癌蛋白阳性病例数量有限，因此不可能将癌基因状态与肿瘤的临床行为相关联。c-erbB-2在鳞状细胞癌中未见表达，在多形性腺瘤中明显表达，尤其是在具有侵袭性生长活性的细胞中。DNA异倍体对恶性肿瘤无特异性，良、恶性病变之间的DNA指数有显著性差异。KM-93抗原在涎腺肿瘤患者血清中的检出率极高，提示检测该抗原对临床有一定的指导意义。

The numerical aberration of chromosome 17 can be detected by fluorescence in situ hybridization in oral tumors and this correlated with malignant behavior of oral tumors. PCNA immunoreactivity correlated with the differentiation and atypia of the tumor cells in squamous cell carcinoma of the oral cavity. PCNA score was significantly higher in the malignant and pre-malignant lesion, and correlate with the degree of dysplasia in pre-malignant lesions. The mean PCNA score decreased significantly after cancer chemotherapy of squamous cell carcinoma. According to this results, the response of cancer cells to anticancer agents may be estimated by consecutive measurement of PCNA. c-erbB-2 oncoprotein is rarely expressed in malignant salivary gland tumors. However, with a limited number of c-erbB-2 oncoprotein positive cases, it was not possible to correlate the oncogene status with clinical behavior of the tumor. c-erbB-2 did not detected in squamous cell carcinoma, but markedly detected in plemorphic adenoma, and especially in the cells which showed invasive growth activity DNA aneuploidy is not specific for malignant tumors and there was significant difference in DNA index between malignant and benign ledsions. KM-93 antigen detected significantly high level in the patient serum of the salivary gland tumotrs, therefore, it seems that measurement of this antigen is useful method for clinical practice.