Kinetics of Kupffer Cells and Cytokines in rejection of Liver Transplantation.

为了阐明Kupffer细胞和细胞因子在肝移植排斥反应中的动力学，采用体外实验系统或体内模型作为大鼠肝移植模型。本实验以Wistar和Lew大鼠为实验动物，研究了脂多糖刺激Kupffer细胞释放细胞因子和肝细胞线粒体ATPase活性的变化。在体外实验中，分离出肝实质细胞(PC)和枯否细胞(KC)，并在相同或不同菌株下进行共培养。在活体实验中。观察KC中Kupffer细胞的分布及分泌IL-1的情况。此外，免疫抑制对上述实验结果的影响如下：(1)在不同菌株下PC与KC共培养的实验。观察到内毒素刺激KC释放细胞因子，PC线粒体ATPase活性下降。(2)环孢素A和类固醇激素(强的松龙和地塞米松)抑制不同品系下PC和KC共同培养促进细胞因子释放的条件，以IL-1、TNF和超氧化物歧化酶为标志。(3)环孢素A和类固醇激素在体内的作用与文献(2)中的结果一致。(4)综上所述，在不同品系的大鼠肝移植中，对KC的关注和细胞因子释放加速条件的抑制作用。

In order to clarify the kinetics of Kupffer cells and cytokines in rejection of liver transplantation, the in vitro experimental system or the in vivo model was utilized as the rat liver transplantation. The release of cytokines from Kupffer cells stimulated by lipopolysaccharide and the activities of hepatocytic mitochondrial ATPase were studied.Animals used in this experiment were Wistar and LEW rats. in the in vitro experiment, hepatic parenchymal cells(PC) and Kupffer cells(KC) were isolated and co-cultured under the same-strains or the different-strains each other. In the in vivo experiment. the distribution of Kupffer cells and the release of IL-1 from KC were studied. Moreover, the effects of the immunosuppressiva on these experimental results were as follows ;(1) In the experiment of the co-culture of PC and KC under the different-strains. the accelerations of cytokines-release from KC stimulated by LPS and the decrease of the activities of PC mitochondrial ATPase were observed. The former results were recongnized as the effects of primings.(2) Cyclosporine A and Steroids (predonisolone and dexamethasone) suppressed the accelerative condition of cytokines-release from KC by the co-culture ofPC and KC under the different-strains, indicating by IL-1, TNF and a superoxide.(3) The effects of cyclosporine A and steroids induced the same significance in vivo as the results in vitro summarized in (2).(4) In summary, the concerning of KC and the suppressive effects of accelerated condition of the cytokines release from KC were suggested in rat liver transplantation under the different-strains.