High-resolution liquid chromatography mass spectrometry system

分子生物学研究所(IMB)是表观遗传学、基因组稳定性和老龄化领域的基础研究机构。IMB配备了最先进的核心设施，为美因茨的整个科学界提供服务。蛋白质组学核心设施(PCF)运行一台高分辨率质谱仪，该质谱仪与纳米流超高效液相系统相耦合。PCF提供的常规服务范围从简单的凝胶条带鉴定到复杂样品的定量分析。目前，它支持无标记、二甲基标记、TMT和基于SILAC的定量蛋白质组实验。如果需要进一步深入的蛋白质组分析，PCF可以提供多种离线蛋白质/多肽分级的选择。它还通过提供内部认证的试剂和测试代谢标记的掺入效率来支持SILAC实验。此外，PCF还可供用户就实验设计、故障排除和生物信息学数据分析提供咨询。PCF的工作人员包括湿实验室和干实验室的科学家，他们拥有广泛的研究经验和技术专长。为了能够继续提供先进的设备，目前已有10年历史的质谱仪必须升级为能够涵盖上述所有应用领域的新的液体色谱质谱系统。

The Institute of Molecular Biology (IMB) is a basic research institute in the field of epigenetics, genome stability, and aging. IMB is equipped with state-of-the-art core facilities, which offer services to the entire scientific community in Mainz. The Proteomics Core Facility (PCF) operates a high-resolution mass spectrometer coupled to a nanoflow UHPLC system. The PCF offers routine services that range from simple gel band identification to quantitative analysis of complex samples. Currently, it supports labelfree, dimethyl-labelled, TMT and SILAC based quantitative proteomic experiments. If further deep proteome analysis is desired, PCF can provide multiple options of off-line protein/peptide fractionation. It also supports SILAC experiments by providing in-house certified reagents and testing incorporation efficiency of the metabolic labelling. In addition, PCF is available to users for consultation on experimental design, troubleshooting and bioinformatics data analysis. The PCF staff consist of both wet- and drylab scientists that possess a broad range of research experience and technical expertise. In order to be able to continue to offer advanced equipment, the current 10-year-old mass spectrometer would have to be upgraded to a new liquid chromatography mass spectrometry system that could cover all the above-mentioned areas of application.