Studies on the genes controlling lethality and development of the silkmoth, Bombyx mori.

我们采用基因组定位作为分析家蚕致死基因的策略，因为我们认为定位克隆是获取致死基因和其他发育控制基因的最直接途径。1. 通过配对实验，构建了22号染色体上rapd、RFLPs和表型位点的综合图谱。这张图谱包含sku致死基因。2. 发育控制基因DH-PBAN被定位在22.3号染色体-2.5cM处。RAPD连锁图谱与7、13和18号染色体作为连锁组的常规表型图谱对应。一些致命的基因，如Obs，是由这些染色体携带的。4. 对Z染色体上的几个rapd进行了克隆和测序。其中一个被认为是单一复制序列。5. 对7号和13号染色体上的部分rapd进行了克隆和测序。8. 结果表明，控制菌株xu20寿命和产卵行为的主要因子与第29个RAPD连锁群上的一个RAPD有关。6. 分析了家蚕和蚕蛾中aryphorin基因同义位点和非同义位点的序列差异。提出了一种基于该基因非同义位点的系统发育树作为家蚕的进化关系。7. 对蓖麻和柞蚕发育过程中及两性间六聚体蛋白信使RNA进行了定量分析。8. 对家蚕核型多角体病毒的无定形多面体突变体和立方多面体突变体的多角体基因进行了测序。我们发现在多面蛋白编码区有几个氨基酸取代。

We adopted genome mapping as a strategy to analyze lethal genes in the silkworm, Bombyx mori since we thought positional cloning would be the most direct pathway to access the lethal genes and other developent-controlling genes. 1. We constructed an integrated map of RAPDs, RFLPs and phenotypic loci on chromosome 22, by mating experiments. This map contains the sku lethal gene. 2. A development-controlling gene, the DH-PBAN gene, was mapped at -2.5cM on chromosome 22.3. The RAPD linkage map was corresponded to the conventional phenotypic map for chromosomes 7,13 and 18 as linkage groups. Some lethal genes such as Obs, are carried by these chromosomes. 4. A few RAPDs on the Z chromosome were cloned and sequenced. One of them was considered to be a single copy sequence. 5. Some RAPDs on chromosomes 7 and 13 were cloned and sequenced. 8. A major factor which control the life span and egg-laying behavior in strain xu20, was determined to be linked to a RAPD on the 29th RAPD linkage group. 6. Sequence divergence at synonymous and non-synonymous sites of the aryphorin gene was estimated from the bombycid and saturniid silkmoths. A phylogenetic tree based on the non-synonymous sites of the gene was proposed as an evolutionary relationship of silkmoths. 7. Messenger RNA for hexamerins were quantitated along develoment and between sexes in Samia cynthia ricini and Antheraea pernyi. 8. The polyhedrin genes of the B.mori nuclear polyhedrosis virus were sequenced for an amorphous polyhedron mutant and a cuboidal polyhedron mutant. We revealed several amino acid substitutions in the polyhedrin-encoding region.