Joint study on oral asaccharolytic Eubacterium species.

解糖真菌种是严格的厌氧革兰氏阳性棒，并且经常从不同的口腔样品中分离和优势细菌种。口腔解糖真杆菌的生长缓慢和氧敏感性阻碍了其在各种口腔传染病中的意义的确定。通过采用高效的厌氧分离方法，我们分离出了几种未分类的真细菌，它们与已建立的真细菌种类不同，即E.brachy， E.lentum， E.nodatum和E.timidum。采用多相法对这些生物的分类位置进行了研究。这些未命名的菌株在常规生化试验中是惰性的，但SDS-PAGE蛋白谱分析和氨基肽酶活性（Rapid ID 32A）结果显示，这些菌株与已建立的解糖菌有很大的不同，并被划分为4个组。在免疫印迹反应和DNA-DNA杂交分析的基础上，提出了产生革兰氏阳性短杆丁酸盐的两组菌株E.saphenum和E.minutum的新种。第三组为苯乙酸产菌，通过其全细胞蛋白谱和氨基肽酶活性的差异可与E.timidum区分开来。第四组菌株产生了微量的苯乙酸酯（如果有的话）。随着厌氧培养和鉴定技术的不断发展和完善，分离出了生长缓慢、培养困难的真细菌。进一步的研究正在进行中，以提供有关未分类菌株和其他真细菌物种在口腔疾病中的作用的信息。

Asaccharolytic Eubacterium species are strictly anaerobic, gram-positive rods and are frequently isolated and predominant bacterial species from different oral samples. The slow growth and oxygen sensitivity of the oral asaccharolytic Eubacterium species has hindered the determination of their significance in various oral infectious diseases. By adopting efficient anaerobic isolation procedures, we isolated several unclassified strains of Eubacterium that were distinct from the established Eubacterium species namely, E.brachy, E.lentum, E.nodatum and E.timidum. A polyphasic approach was used to examine the taxonomic positions of these organisms. These un-named strains were inert in conventional biochemical tests, however, the results of SDS-PAGE protein profile analysis and aminopeptidase activty (Rapid ID 32A) revealed that these strains were quite distinct compared from the established asaccharolytic species and assigned to four groups. Two groups of strains, is butyrate producing gram-positive short rods, have been proposed new species namely, E.saphenum and E.minutum, established on the additional basis of western immunoblotting reactions and DNA-DNA hybridization analysis.The third group were phenylacetate producing strains, which can be distinguished from E.timidum by the differences in whole-cell protein profile and aminopeptidase activity. The strains in the fourth group produced a trace (if any) of phenylacetate. The continuos development and improvement of anaerobic incubation and identification techniques resulted in the isolation of slow growing and culture-difficult asaccharolytic species of Eubacterium. Further studies are in progress to provide information on the roles of the unclassified strains and other Eubacterium species in oral disease.