Improvement of plant propagation in tropical problem-soils

与泰国Kasesart大学合作，对泰国东北部盐渍化地区的杂草进行了调查，目的是收集有益的遗传资源，以改善一年中有旱季和雨季的热带季风区问题土壤中的植被。利用耐盐杂草进行繁殖技术的开发和耐盐强化植株的选择。此外，还对收集到的植物进行了生物化学研究，以阐明耐盐和盐害的机理，并评价其对盐渍土壤的绿化效果。耐盐杂草调查：调查了7个省（Nakhonrachasima、Mahasarakam、Khon Kaen、Skonnakhon、Udonthani、Surin和Srisaket） 17个地区的杂草和土壤特征。在EC值大于2mS/cm的高盐度土壤中，共鉴定出33科132种，27科79种。耐盐植株的选择：从香根草（Vetivara zizani coides Nash）植株诱导的愈伤组织在1.5%盐浓度下仍表现出较高的耐盐性。再生成功，并在盐碱地中进行了试验，符合耐盐性的维持。3 .耐盐机理：通过对盐的吸收和分布、相容溶质、活性氧清除系统等方面的研究，比较了杨梅和籼稻品种耐盐型和盐敏感型的差异，阐明了其生物化学机制。耐盐杂草对盐碱地的适应性：在Kasesart大学盐渍地对收集到的耐盐胁迫杂草进行试验，并收集数据，评价草本杂草恢复植被的有效性以及对土壤特征等微环境的影响。

Survey of weeds in the salinized areas in Northeast Thailand was conducted with cooperation of Kasesart University for the purpose of collecting the useful genetic resources to improve the vegetation in the problem soils of the tropical monsoon areas which have the dry and rainy seasons in a year. Salt-tolerant weeds were utilized to develop the propagatinal technique and to select the fortified plants in salt tolerance. Furthermore, the collected plants were studied for clarifying the mechanism of salt tolerance and salt damage biochemically and evaluating the effectiveness in greening the salt-affected soil.1.Survey of weeds tolerant to salinity : Weeds and soil characteristics of 17 areas of 7 provinces (Nakhonrachasima, Mahasarakam, Khon Kaen, Skonnakhon, Udonthani, Surin, and Srisaket) were surveyed. Totally, 33 families and 132 species were identified and 27 families and 79 species of them were remained alive at high salinity soils more than 2mS/cm in EC value.2.Selection of salt-tolerant plants : Some of the calli induced from vetivar Vetivara zizani coides Nash) plant showed high salt tolerance even under 1.5% salt concentration. They were regenerated successfully and tested in the salinity soil to conform the maintenance of salt tolerance.3.Mechanism of salt tolerance : Comparison between salt-tolerant and salt-sensitive biotypes of Imperata sylindrica and indica and japonica rice varieties, respectively, were conducted to clarify the biochemical mechanism by investigating the absorption and disrtibution of salt, compatible solutes, and scavenging system of activated oxygen.4.Adaptibility of salt-tolerant weeds to salinity soil : The collected weeds tolerant to salt stress were tested in the salt-affected field of Kasesart University and data are collecting to evaluate the effectiveness of herbaceous weeds in restoring vegetation and influence on the micro-envirnment including soil characteristics.