NU-UCB Cooperative Program on plant Molecular Biology

名古屋大学（NU）和加州大学伯克利分校（UCB）的成员和非成员在“NU-UCB植物分子生物学合作计划”下进行了合作和积极讨论，取得了以下进展。1)汇活性和钙信号的调控项目重点研究的课题之一是钙信号在汇源相互作用调控中的作用。Nakamura和Sugiyama （NU）已经表明钙信号分别参与糖诱导基因表达和硝酸盐调控基因表达。Jones （UCB）发现大麦种子淀粉粉层的功能依赖于钙调蛋白的特定异构体，Nakamura从烟叶中部分纯化了一种与质膜紧密相关的钙依赖性蛋白激酶（CDPK）的新异构体。Sugiura （NU）和Grulissem （UCB）通过体外系统研究叶绿体基因的表达，Sugiura建立了体外翻译系统，Gruissem建立了体外转录物成熟系统。maeshima （NU）和Jones （UCB）合作研究了Jones在大麦种子糊粉细胞中发现的新型液泡样结构的免疫细胞化学特征。Maeshima分离到了叶绿体Ca^<2+>/H^+反转运蛋白的cdna。4)拟南芥分子遗传学研究：Fischer （UCB）从T-DNA标记的突变系中发现Bell-1编码胚胎发生必需的同源结构域因子，Machida （NU）发现nadph氧化酶在生长控制中起重要作用。Machida还发现了一个损伤后快速激活的质膜相关激酶，Gruissem （UCB）分离到了一个参与肌醇-磷酸代谢的基因。5)农业应用：akamura和Machida （NU）帮助Buchanan和Lemaux （UCB）利用NU开发的载体开始了农杆菌介导的大麦转化。少

Collaboration and stimulative discussions among members, and non-members, of the Nagoya University (NU) and the University of California, Berkeley (UCB) under the program "NU-UCB Cooperative Program on Plant Molecular Biology", yielded the following progress.1) Regulation of sink activity and calcium-signalingOne of the reseach topics focused in the program was the role of calcium-signaling in the regulation of sink-source interaction. Nakamura and Sugiyama (NU) have shown that calcium-signaling is involved in the sugar-inducible gene expression, and in the nitrate-regulation of gene expression, respectively. Jones (UCB) found that function of aleurone layr of barley seeds depends on specific isoform of calmodulin, and Nakamura partially purified a novel isoform of calcium-dependent protein kinase (CDPK) from tobacco leaves which is tightly associated with the plasma membrane.2) Regulation of source activity and plastid gene expressionSugiura (NU) and Grulissem (UCB) communicated on th … More e in vitro systems to study chloroplast gene expression, and Sugiura developed in vitro translation system and Gruissem developed in vitro system for the maturation of transcripts.3) Function of plant vacuolesMaeshima (NU) and Jones (UCB) collaborated on the immuno-cytochemical characterization of novel vacuole-like structures which Jones found in aleurone cells of barley seeds. Maeshima isolated cDNAs for tonoplast Ca^<2+>/H^+ antiporter.4) Molecular genetics of Arabidopsis thalianaFrom T-DNA tagged mutant lines, Fischer (UCB) found that Bell-1 codes for a homoeodomain factor essential for embryogenesis, and Machida (NU) found that NADPH-oxidase plays an important role in the growth control. Machida also identified a plasma membrane-associated kinase which is rapidly activated after wounding, and Gruissem (UCB) isolated a gene involved in the metabolism of inositol-phosphate.5) Application to agricultureNakamura and Machida (NU) helped Buchanan and Lemaux (UCB) to start Agrobacterium-mediated transformation of barley by vectors developed in NU. Less