New method of measuring CDNA by PCR and

逆转录（RT）和聚合酶链反应的组合可用于分析低水平的mRNA，但有时不能产生定量信息，特别是当靶mRNA的量非常小时。一些研究人员描述了竞争性聚合酶链反应，其中含有与靶相同的引物模板序列的DNA片段竞争引物结合和扩增。在这里，我们开发了一种方法来检查小鼠催乳素受体cDNA的短长形式的比例，通过“单侧竞争性聚合酶链反应”。该程序由聚合酶链反应组成，使用两种形式共有的引物和它们各自特异性的引物。此外，使用竞争性聚合酶链反应开发了测量编码催乳素受体胞外结构域的cDNA水平的方法，以估计cDNA样品中催乳素受体cDNA的量。本研究试图建立一种组织切片上mRNA的检测方法。

A combination of reverse transcription (RT) followed by the polymerase chain reaction is useful for analyzing low levels of mRNAs, but it sometimes does not yield quantitative information especially when the amount of the target mRNA is very small. Some researchers have described competitive polymerase chain reaction in which DNA fragments containing the same primer template sequences as the target compete for primer binding and amplification. Here, we developed a means of examining the ratio of the short to the long form of mouse prolactin receptor cDNAs, by means of "one-sided competitive polymerase chain reaction" . The procedure consisted of polymerase chain reaction using a primer common to both forms and primers specific to each of them. In addition, a means of measuring the level of cDNA encoding the extracellular domain of prolactin receptor was developed using competitive polymerase chain reaction, to estimate amount of prolactin receptor cDNA in cDNA samples. Now we try to develop a detection method for mRNA on the tissue section.