Mechanisms of proliferation and migration of human vascular smooth muscle cells

首次成功的经皮腔内冠状动脉成形术（PTCA）后再狭窄发生在30-40%的患者中，因此仍然是一个重要的临床问题。在绝大多数情况下，以平滑肌细胞为主要细胞成分的纤维细胞组织反应是再狭窄的潜在形态学底物。平滑肌细胞的迁移和增殖以及细胞外基质成分的合成与其细胞骨架特征密切相关。迄今为止，大多数信息都是从实验研究中获得的，人类平滑肌细胞迁移和增殖的机制尚不清楚。本研究首次证实PDGF-B和pdgge - a链mrna在PTCA后的冠状动脉修复位点均有表达（Ueda M等）。Am J Pathol 1996)。该研究还显示PDGF-B和pdgf - β受体蛋白在人类血管成形术后修复部位的表达（Tanizawa S等）。心1996)。这些发现强烈提示PDGF参与了人冠状动脉平滑肌细胞的迁移和增殖过程。此外，目前的研究表明，腱素的上调可能在人类平滑肌细胞的迁移和表型调节中发挥作用（Tanabe S等）。译于1996年)。本研究还首次证实，c型利钠肽（CNP）是一种有效的平滑肌细胞抑制剂，在人冠状动脉内膜细胞中表达，并在动脉粥样硬化中具有功能意义（Naruko T et al.）。发行量1996)。最后，我们的研究表明，在PTCA位点的平滑肌细胞迁移和增殖过程中，平滑肌细胞表现出未分化状态的特征，这可以通过平滑肌肌球蛋白重链的表达改变来体现（Aikawa M et at.）。（报刊发行量）。

Restenosis after an initial successful percutaneous transluminal coronary angioplasty (PTCA) occurs in 30-40% of patients and thus remains a significant clinical problem. In the vast majority, a fibrocellular tissue reaction with smooth muscle cells, as the prime cellular component, is the underlying morphologic substrate of restenosis.Migration and proliferation of smooth muscle cells as well as synthesis of extracellular matrix components, relate closely to their cytoskeletal features. Most information thus far has been obtained from experimental studies, and the mechanisms that underlie migration and proliferation of human smooth muscle cells are unclear still.The present study demonstrates, for the first time, that both PDGE-A and PDGF-B chain mRNAs are expressed at sites of repair in human coronary arteries after PTCA (Ueda M et al. Am J Pathol 1996). This study also shows the expression of PDGF-B and PDGF-beta receptor proteins at sites of postangioplasty repair in humans (Tanizawa S et al. Heart 1996). These findings strongly suggest that PDGF is involved in the process of migration and proliferation of smooth muscle cells in human coronary arteries.The present study, moreover, documents that up-regulation of tenascin may play a role in the migration and phenotypic modulation of smooth muscle cels in humans (Tanabe S et al. Transpl Int 1996). The present study also demonstrates, for the first time, that C-type natriuretic peptide (CNP), which is a potent inhibitor of smooth muscle cells, is expressed in intimal cells of human coronary arteries, and has functional significance in atherogenesis (Naruko T et al. Circulation 1996). Finally, our study shows that, during smooth muscle cell migration and proliferation at the site of PTCA,smooth muscle cells show features of an undifferentiated state, indicated by altered expression of smooth muscle myosin heavy chain (Aikawa M et at., Circulation in press).