The evaluation of physical exercise by stress protein.

体育锻炼的生理生化评价尚不明确。体育锻炼会产生各种刺激，这取决于锻炼的强度和持续时间。近年来，在基础科学和医学领域，对各种应激诱导的应激蛋白的研究越来越多。本研究以大鼠为实验对象，在跑台上进行运动实验，探讨运动中补充应激蛋白的适宜性。在DNA和RNA表达水平上，利用原位DNA杂交和原位杂交研究应激蛋白的诱导。此外，还测定了运动后的血乳酸和血糖浓度。由于运动后血乳酸浓度升高到较高水平，而血糖浓度降低到较低水平，因此本研究中使用的运动强度相当高。结果发现，运动后大鼠肾脏中HSE-HSF的结合比对照组增加。原位杂交结果显示，运动后大鼠皮肤、脾脏、肾脏、肝脏和心脏中HSP 70 mRNA的诱导表达均较对照组增加。但在大鼠骨骼肌（比目鱼肌、跖肌、趾长伸肌）中HSP 70 mRNA的表达量无明显增加。从这些结果来看，应激蛋白的表达似乎可用于体育锻炼的生化评价。此外，原位杂交是检测运动诱导HSP的有效方法。

The physiological and biochemical evaluation of the physical exercise is not clear. Physical exercises induces various stimuli depending on exercise intensity and duration. Recently stress protein, which is induced by the various stress, is well studied in the field of basic and medical sciences. In the study, we examined the propriety of stree protein for the physical exercise.We did experiments using rats on treadmill running. The induction of stress proteins was investigated at the level of DNA and RNA expression, using in situ DNA hybridization and in situ hybridization. In addition, blood lactate and blood glucose concentrations after exercise were measured. Since the blood lactate concentration increased to high level and the blood glucose concentration decreased to lower level after exercise, the exercise intensity used in this study was rather high. As a result, we found that the binding of HSE-HSF was increased in rat kidney after exercise with in situ DNA hybridization, compared with control group. The induction of HSP70mRNA with in situ hybridization after exercise increased in rat a dernal, spleen, kidney, liver and heart compared with control group. However the induction of HSP70mRNA didn's increase in rat skeletal muscle (soleus, plantaris, EDL). From these results, the expression of stress protein seems to be available for the biochemical evaluation of physical exercise. In addition, in situ hybridization is an effective method for the detection of HSP induction by exercise.