Analysis of sweating of human skin transplanted on the weak immune mouse

异种人皮肤移植物提供了一种新的模式，允许独立于正常的汗腺神经支配，分析汗腺细胞的分子受体，自然递质和药理药物的作用由其传导。(1) 1999年的实验结果与1998年实验系列I（16个实验，16个受试者）的实验结果相结合。将100mg/ml标准浓度乙酰胆碱离子灌注与尼古丁、匹洛卡品、肾上腺素和血管活性肠多肽（VIP） 3种浓度（0.06、0.36、1.10 mg/ml）进行比较。胆碱能激动剂可诱导直接出汗反射（DIR）和压迫运动轴突反射（AXR）。肾上腺素可诱导微弱的直接出汗反射（DIR），但不能诱导AXR。VIP浓度升高时，DIR未产生AXR，皮肤温度略有升高。(2)实验系列二（6个实验，6个受试者）。第一次VIP离子导入（0.36mg/ml）后，引起DIR，但未引起AXR。受试者进行30分钟的运动以诱导出汗。休息60分钟后，出汗消失，心率正常，但体温仍然升高（口腔温度升高0.22℃，皮肤温度升高0.67℃），表明体温调节有利于散热。VIP离子导入现在引起局部皮肤温度的较大升高，引发微弱但明显的AXR，潜伏期约为2.5 min， DIR增强。(3)高场微成像具有灵敏度高、空间分辨率高的优点，T1和T2加权图像对比度好，可作为小鼠生理病理研究的标准动物模型。

The heterologous, human skin graft provides a novel moded permitting, independent of the normal sweat gland innervation, the analysis of molecular receptors of sweat gland cells by which the actions of natural transmitters and pharmacological agents are transduced.(1) The experimental results of 1999 combined with these of 1998Experimental series I (16 experiments on 16 subjects). Acetylcholine iontophoresed at 100mg/ml standard concentration was compared with nicotine, pilocarpine, adrenaline and with vasoactive intestinal polypeptide (VIP) at 3 different concentrations (0.06, 0.36, 1.10 mg/ml). Cholinergic agonists induced both direct sweating reflex (DIR) and sudomotor axon reflex (AXR) Adrenaline induced a weak DIR but no AXR. With the 2 higher VIP concentrations DIR but no AXR was produced, and skin temperatures increased slightly.(2) Experimental series II (6 experiments on 6 subjects). After a first VIP iontophoresis at (0.36mg/ml) which elicited DIR but not AXR. The subjects exercised for 30 min to induce sweating. After 60 min rest , sweating had disappeared and heart rate was normal, but temperatures remained elevated (oral temperature by 0.22℃, skin temperatures by 0.67℃) indicating a thermoregulatory stated favoring heat dissipation. VIP iontophoresis now caused larger rises of local skin temperature, elicited weak but distinct AXR with a latency of about 2.5 min and an augmented DIR.(3) High-field micro imaging has advantages of higher sensitivity and higher spatial resolution, and T1- and T2- weighted images offer good contrasts which is able to use mouse as a standard animal model for physiological and pathological studies.