Induction mechanism of and protection mechanism against the peroxidation of lipids in plant cells.

铝（Al）是一个主要因素，以抑制植物根生长在酸土壤中的作用。铝毒性的分子机理和其他铝耐受性在文化烟草细胞和豌豆根部进行了研究。在含有营养介质铁离子（II， III）的烟草细胞中，通过刺激脂质中的铁介导的过氧化物作用，包括DNA的核间细胞破裂。两种类型的高耐受性烟草细胞线是抗耐受性表型的隔离和受调查的特性。Since they showed Tolerance also to various kinds of reagents （HイD22イD2OイD22イD2， t-butyl hydroperoxide， A23187） which induce the peroxidation of lipids， it seems that they acquired a Tolerant Mechanism against the peroxidation of lipids。关于细胞线累积表态学，主要的一个被鉴定为LC/MS和NMR的咖啡酰分泌物。Caffeoyl putresine受保护的烟草细胞，由Al和Fe的组合引起的脂质过氧化物（II）。本单元格还展示了一种类似于动物系统中谷氨酰胺过氧化物酶的酶活性，在豌豆根上，也增强了脂质的过氧化物酶，但不需要铁的协同作用。脂质的老化是Al引起的早期事件之一。脂质的铝增强老化并不是由于在Al治疗期间对根生长的抑制，但部分原因是由于对游离介质中根再生长的抑制。该结果表明，脂质在两种文化植物细胞和根中的老化，并提出这样的抗氧化剂，如卡菲伊尔-普雷斯汀和谷氨酰胺过氧化物酶，似乎可以保护植物细胞从脂质的强化过氧化物中的作用。

Aluminum (Al) is thought to be a major factor to inhibit plant root growth in acid soils. Molecular mechanisms of Al toxicity and also of Al tolerance were investigated in cultured tobacco cells and pea roots.In tobacco cells in nutrient medium containing Fe ion (II, III), Al stimulated the Fe-mediated peroxidation of lipids which caused apoptosis-like cell death including internucleosomal breakage of DNA. Two-types of Al-tolerant tobacco cell lines were isolated and investigated specificity of the tolerance phenotype. Since they showed tolerance also to various kinds of reagents (HィイD22ィエD2OィイD22ィエD2, t-butyl hydroperoxide, A23187) which induce the peroxidation of lipids, it seems that they acquired a tolerant mechanism against the peroxidation of lipids. ON of the cell line accumulated phenolics, and the major one was identified as caffeoyl putresine by LC/MS and NMR. Caffeoyl putresine protected tobacco cells from the peroxidation of lipids cause by a combination of Al and Fe (II). This cell line also exhibit an enzymatic activity similar to glutathione peroxidase in animal systems.In pea roots, Al also enhanced the peroxidation of lipids, but did not require the coexistence of Fe. The peroxidation of lipids was one of the early events caused by Al. The Al-enhanced peroxidation of lipids was not cause of the inhibition of root elongation during Al treatment, but was partly a cause of the inhibition of root re-growth in Al-free medium.The results indicate that Al enhances the peroxidation of lipids in both cultured plant cells and roots, and suggest that antioxidants such as caffeoyl putresine and glutathione peroxidase seem to protect plant cells from the Al-enhanced peroxidation of lipids.