STUDY AS TO INTERACTION BETWEEN IMMUNOLIPOSOME AND VASCULAR ENDOTHELIAL CELLS IN VIVO

为了研究免疫脂质体与血管内皮细胞在体内的相互作用，将脂质体化学加工成内含金、铁、铜颗粒的免疫脂质体和外带抗体的聚乙二醇单抗体(ICAM-1或VCAM-1)。它们的直径约为100 nm，表面约有30个分子，其中30%包括金属。事先证实，制备的免疫脂质体通过放射性同位素聚集在肝脏和肠系膜淋巴结内。他们被注射到小鼠和大鼠的静脉中。1h后灌流固定，做扫描电镜和透射电子显微镜观察。在肝脏中，免疫脂质体结合到筛板两侧周围的区域。肝窦内皮细胞通过内吞作用摄取免疫脂质体，这些免疫脂质体集中在内吞体内。在淋巴结内，免疫脂质体附着在高内皮微静脉(HEV)上，与淋巴细胞归巢有关，尤其是靠近交界处的狭窄空间。这些结果总结如下：(1)脂质体易于加工成含有金属的免疫脂质体。(2)在体内表现出抗原在表面的某些行为。(3)在活体内提供了将抗原复合体摄取到细胞质中的信息。最后，体内注射免疫脂质体是研究体内血管内皮细胞表达抗原的细胞生物学动力学的有用工具。

To investigate interaction between immunoliposomes and vascular endothelial cells in vivo, liposomes were processed chemically into immunoliposomnes, which contained Au, Fe, or Cu particles in the interior and Polyethyleneglycol with anibody (ICAM-1orVCAM-1) outside. They were approximately 100nm in diameter and had about 30molecules on the surface, 30% of which included metals. It was confirmed in advance that prepared immunoliposomes gathered in the liver and mesenteric lympnodes with a radioisotope. They were injected into the veins of mice and rats. After 1hour they were fixed with perfusion fixation and prepared for SEM and TEM. In the liver, immunoliposomes bound to the area around the both sides of the sieve plate. Hepatic sinusoid endothelial cells ingested immunoliposomes by endocytosis, which were concentrated into the endosomes. In the lymph node, immunoliposomes adhered to high endothelial venuls (HEV), which were associated with lymphocyte homing, especially the narrow space near the junction. These results were summarized as follows:(1)Liposomes are processed easily into immunoliposomes containing metals.(2)They show some behavior of antigens on the surface in vivo.(3)They give intracellular information of ingesting antigen complex into the cytoplasm in vivo.Finely an immunoliposome injection in vivo is a useful tool to investigate cell biological dynamics of antigen express on vascular endothelial cells in vivo.