Role of Nitric Oxide on Minimum Alveolar Anesthetic Concentration

“7-硝基吲唑对氟烷最低肺泡麻醉浓度的影响”方法：SD大鼠14只，随机分为7-硝基吲唑(7-NI；选择性神经元型一氧化氮合酶抑制剂)组和对照组。测定注射7-NI(100 mg/kg)或赋形剂前后氟烷的最低肺泡麻醉浓度(MAC)。结果：7-NI可使蓝斑(LC)和脊髓(SC)的nNOS活性降低约50%，并使SC和SC的nNOS表达减少约25%。用NADPH-黄递酶组织化学方法研究氟烷MAC和NADPH-黄递酶组织化学变化。结果：团注地塞米松使LC和SC的MAC减少约50%，但不改变NADPH-黄递酶组织化学。地塞米松持续给药不会降低MAC，但DEX给药3天组大鼠腰椎Lc和IV-V椎板nNOS活性下降，而DEX给药2周组则无明显变化。结论：一氧化氮(NO)在MAC的决定中起重要作用，但NO不是唯一因素。

"Effects of 7-Nitro Indazole on Halothane Minimum Alveolar Anesthetic Concentration"Methods : Fourteen Sprague-Dawley rats were divided into 7-Nitro indazole (7-NI ; selective neuronal nitric oxide synthase inhibitor) group and control group. We measured minimum alveolar anesthetic concentration (MAC) of halothane before and after injection of 7-NI (100 mg/kg) or vehicle. Using NADPH-diaphorase histochemistry, we also investigated the nNOS activity of the locus ceruleus (LC) and the spinal cord (SC).Results : 7-NI reduced the MAC about 50 %, which was accompanied by about 25 % suppression of nNOS staining in SC and LC."Halothane Minimum Alveolar Anesthetic Concentration and NADPH-diaphorase histochemistry after Dexmedetomidine injection"Methods : Thirteen-six Sprague-Dawley rats were divided into three Dexmedetomidine (DEX) groups (50μg/kg bolus, 50μg/kg/day ; three-day and two-week continuous administration) and three control groups (vehicle). We investigated halothane MAC and the nNOS activity of the LC and the SC using NADPH-diaphorase histochemistry.Results : Bolus injection of DEX reduced the MAC about 50 %, however, did not change the NADPH-diaphorase histochemistry. On the other hand, continuous administration of DEX did not reduce the MAC, however, nNOS activity of the LC and IV-V laminae of the lumbar SC decreased in the three-day DEX group, but not the two-week DEX group.Conclusion : Nitric oxide (NO) plays an important role in decision of MAC, however, NO is not only one factor.