Studies for the mechanism of adrenal hypertrophy induced by toluene inhalation, and endocrinological changes in adrenal gland

甲苯是稀释剂的主要成分，稀释剂是年轻人滥用药物的中心。众所周知，甲苯对大脑具有很高的亲和力，并会出现各种神经和心理症状。本研究观察了甲苯吸入对肾上腺的影响。选用Wistar雄性大鼠。这只老鼠被要求每天吸入甲苯(1000ppm)四个小时。分别于雾化吸入后4、7、10天与对照组进行对比观察。结果表明，甲苯吸入可引起大鼠肾上腺肥大，尤其是肾上腺皮质肥大。血清ACTH水平无显著差异。然而，有人认为丘脑-垂体-肾上腺系统与肾上腺肥大有关。预测肾上腺皮质内分泌功能改变是以肾上腺皮质肥大为基础的。但肾上腺皮质激素合成量和血清醛固酮的量无显著差异。即使存在激素的过度合成和分泌，这种改变也被认为是一种早期的暂时性改变，而且神经营养因子GDNF的高表达被认为是肾上腺髓质的改变。用免疫组织化学方法检测脊髓神经元和肾上腺髓质嗜铬细胞中HSP70和c-Fos的表达。认为GDNF在肾上腺髓质中的表达是由于脊髓神经元的损伤所致。提示甲苯吸入可引起脊髓作为中枢神经系统的一部分神经元损伤。

The toluene is a principal ingredient of thinner that is the center of youth's abuse medicine. The toluene is well known to be high affinity for the brain and to present a various neurological and psychological symptoms. In this research, changing the adrenal by the toluene inhalation was examined. Wistar male rats were used. The rat was made to inhale toluene (1,000 ppm) for four hours a day. We examined for the inhalation group ; 4, 7 and 10 days of the inhalation against control group. As a result, the toluene inhalation induced adrenal hypertrophy, especially adrenal cortex. A significant difference was not admitted in serum ACTH level. However, it was suggested that the thalamus- pituitary - adrenal system related adrenal hypertrophy. It was forecast that the change in adrenal cortical internal secretion function took place based on adrenal cortical hypertrophy. However, the amount of mRNA of the adrenocortical hormone synthesis and serum aldosterone did not indicate significant. It was thought that the change stayed in an early temporary change even if there was hyper- synthesis and secretion of the hormone.Moreover, hyperexpression of GDNF, which was neurotrophic factor, was taken as changing adrenal medulla. We examined HSP70 and c-Fos in the neuron of spinal cord and in the chromaphine cell of adrenal medulla immunohistochemically. It was thought that the GDNF expression in adrenal medulla appeared due to the neuronal damage of spinal cord. This result suggested that toluene inhalation induce neuronal damage in spinal cord as a part of the CNS.