A role of sulfated carbohydrate chain in carcinogenesis of Barrett's esophagus and development of enzymeimmunoassay for sulfomucin.

巴雷特食管（BE）是一种获得性疾病，与腺癌的高发病率有关。本研究的目的是确定BE的患病率和BE中的粘蛋白谱，包括短节段型，并将BE中的粘蛋白谱与Barrett癌的粘蛋白谱进行比较。共650例接受内镜检查的BE患者，虽然长型BE的患病率为0.62%，但包括短型BE在内的总患病率为15.7%。在BE中，短型以胃型粘蛋白为主，而中型和长型以肠型粘蛋白为主，尤其是结肠型粘蛋白（sulfo-Lewis^a）。在伴有腺癌的BE中（8例），所有邻近癌的巴雷特上皮均表现出对磺基刘易斯a的免疫反应性。在Barrett癌中，100%检测到结肠型粘蛋白（sulfo-Lewis^a）。小肠粘蛋白（唾液酸Tn）和胃粘蛋白（MUC 5AC，MUC 6）分别在50%和12.5%的受试者中染色。在5例BE癌组织中，基质金属蛋白酶-7在癌组织前端呈阳性表达，并与结肠粘蛋白共表达。酪蛋白酶谱分析显示基质金属蛋白酶-7的分子量为19 kd，为活化型。E-选择素是磺基-Lewis ^a的有效配体，其表达与结肠粘蛋白之间没有明确的关系。P53在所有BE癌中均过表达。使用微卫星标记BAT 26的微卫星不稳定性在BE癌中未检测到。这些数据表明，表位，而不是小肠或胃型粘蛋白，但结肠粘蛋白（磺基-路易斯α），可能与BE的致瘤性和这种肿瘤的进展。

Barrett's esophagus (BE) is an acquired disorder associated with a high incidence of adenocarcinoma. The object of this study was to define the prevalence of BE and mucin profile in BE, including the short segment type, and to compare the mucin profile in BE with that of Barrett's carcinoma. In total 650 cases underwent endoscopic study for evaluation of BE.Although the prevalence of long BE was 0.62%, the overall prevalence of BE including short type was 15.7%. In BE, the short type predominantly had gastric type mucin, while the middle and long types possessed intestinal mucin, especially colonic mucin (sulfo-Lewis^a). In BE with adenocarcinomas (8 cases), all Barrett's epithelium adjacent to carcinomas showed a predominance of immunoreactivity to sulfo-Lewis a. In Barrett's cancer, colonioc type mucin (sulfo-Lewis^a) was detected in 100%. Small intestinal mucin (sialyl Tn) and gastric mucin (MUC5AC, MUC6) were stained in 50% and 12.5% of subjects, respectively. Matrixmetalloproteinase-7 were detected at the front of the carcinoma tissues in five BE cancer and this enzyme was coexpressed with colonic mucin. Cazein zymography showed that molecular weight of matrixmetalloproteinase-7 was 19kd indicating activating type. There was no clear-cut relation between the expression of E-selectin, which is a potent ligand for sulfo-Lewis^a, and colonic mucin. P53 over expression was proven in all BE cancers. Microsatellite instability using microsatellite marker BAT26 was not detected in BE cancers. These data suggested that the epitope, not of small intestinal or gastric type mucin, but of colonic mucin (sulfo-Lewis^a), may be associated with both tumorigensis of BE and progression of this tumor.