Enhanced antitumor effect of ultrasound in the presence of piroxicam in a mouse airpouch model.

采用小鼠气袋模型，观察超声照射下非甾体抗炎药吡罗昔康对180肉瘤细胞的抗肿瘤作用。在超声（2 MHz, 10 W, 120 s）照射下，将吡罗昔康加入肉瘤180悬液中，照射后肿瘤细胞的立即死亡率和小鼠存活率均显著高于超声单独照射时，且吡罗昔康的这些作用具有剂量依赖性。李氏汀与吡罗昔康联合使用时，照射后肿瘤细胞的死亡率与单独应用超声时相当，同时使用d -甘露醇时，抗肿瘤效果明显大于单独应用超声时。对气袋及周围组织的组织学检查显示，气袋内高度退行性肿瘤组织，周围组织外观正常。研究结果表明，吡罗昔康通过增加单线态氧的产生而不损害肿瘤周围组织，从而增强了超声在体内的抗肿瘤作用。

The antitumor effects of piroxicam, a non-steroidal anti-inflammatory drug, under ultrasonic irradiation on sarcoma 180 cells were examined in a mouse air pouch model. When piroxicam was added to sarcoma 180 suspension under the ultrasound irradiation (2 MHz, 10 W, 120 s), the mortality rate of tumor cells immediately after the irradiation and the survival rate of mice were significantly higher than those when ultrasound alone was applied, and these effects of piroxicam were dose-dependent. When Lhistidine was used with piroxicam, the mortality rate of the tumors cells after the irradiation was comparable with that when ultrasound alone was applied, and when D-mannitol was used concurrently, the antitumor effect was significantly greater than that when ultrasound alone was applied. Histological examinations of the air pouch including the surrounding tissue showed highly degenerative tumor tissue in the air pouch and normal appearance of surrounding tissue. The findings suggest that piroxicam enhances the antitumor effects of ultrasound in vivo by increasing the production of singlet oxygen without any damage to tissue surrounding the tumor.