Functional role of the spiral ligament related to the conchiear recovery

1. 硫酸二氢链霉素损伤小鼠耳蜗螺旋韧带的细胞增殖研究发现，硫酸二氢链霉素损伤的耳蜗螺旋韧带中有许多BrdU标记的细胞核。在BrdU标记细胞的螺旋韧带中检测到成纤维细胞生长因子受体和连接蛋白43的表达。提示药物损伤后螺旋韧带内可能发生细胞增殖，这一过程可能与耳蜗功能恢复有关。渗透泵硫酸链霉素化学迷路切除术的有效性和应用采用30%硫酸链霉素渗透泵化学迷路切除术。治疗后，两组自发性眼球震颤发生频率及偏航头倾斜程度差异无统计学意义。这些数据表明了渗透泵的可靠性和有效性，并且有可能利用渗透泵制作豚鼠模型来预测人类前庭周围不同程度的损伤。酸性成纤维细胞生长因子在听觉创伤恢复中的作用我们通过评估听觉脑干反应（ABR）阈值来评估耳蜗功能，并使用共聚焦激光扫描显微镜观察感觉上皮。噪声暴露（120dB SPL, 5h）后，ABR阈值升高~ 50dB SPL， 14d后恢复。aFGF组耳蜗功能恢复速度快于对照组。提示aFGF可能在听力学损伤后耳蜗恢复中起重要作用。

1. Cell proliferation in spiral ligament of mouse cochlea damaged by dihydrostreptomycin sulfateMany BrdU labeled nuclei in the spiral ligament in the conchlea damaged by dyhydrostreptomycin sulfate were found. The expressions of fibroblast growth factor receptor and connexin 43 were detected in the spiral ligament where BrdU labeled cells were found. These results suggest that the cell proliferation in the spiral ligament may occur after the drug-induced damage, and this process is probably related to the recovery of the cochlear function.2. Effectiveness and utility of chemical labyrinthectomy with streptomycin sulfate using osmotic pumpLabyrinthectomy was performed surgically or chemically with 30% streptomycin sulfate using osmotic pumps in guinea pigs. After treatment, no statistical differnce was observed in the frequency of spontaneous nystagmus and the degree of yaw head tilt between the groups. These data indicate the reliability and efficacy of osmotic pumps, and it might be possible to make guinea pig models using osmotic pumps to predict various grades of damage in the vestibular periphery of humans.3. The role of acidic fibroblast growth factor in recovery of acoustic traumaWe assessed auditory brain stem response (ABR) thresholds to evaluate cochlear function and observed the sensory epithelium using confocal laser-scanning microscopy. After noise exposure (120dB SPL, 5h), the ABR threshold showed an increase of 〜50dB SPL that recovered after 14 days. Cochlear function in aFGF treated ears recovered more quickly than that in control ears. These results suggest that aFGF may play an important role in cochlear recovery after acoustic trauma.