Morpho-functional study of trigeminal neurons in the brain stem that are involved in oro-facial sensori-motor function.

C-Fos是DNA结合蛋白的一员，电刺激三叉神经节后，三叉神经感觉核神经元中c-Fos表达上调。其表达随刺激强度的增加而增强，阳性神经元呈核特异性分布。在尾侧亚核(SpVc)，多数标记神经元位于背内侧腹外侧轴的浅层。在口腔亚核，c-Fos阳性神经元仅出现在背内侧亚核。低强度刺激(0.1 mA)激活A-β纤维和高强度刺激(1.0 mA)也激活C-纤维所诱导的c-Fos表达受预先给予GABAA和GABAB受体激动剂和拮抗剂的不同调节。三叉神经感觉核的节段性和棘上强度依赖性的GABA能调节作用已被证实。鞘内注射c-fos反义寡核苷酸…佐剂减少可诱导CFos表达，延长热痛敏，但不影响正常的伤害性感觉。因此，伤害性感觉后c-Fos的表达参与了痛觉过敏的维持和/或发展。大脑皮层躯体感觉区的电刺激被应用于许多患有顽固性疼痛的患者，疼痛缓解效果良好到非常好。我们证明，电刺激大脑皮层的次级感觉区显著减少了福尔马林诱导的SpVc中c-Fos的表达。神经元型一氧化氮合酶(n-NOS)和烟酰胺腺嘌呤二核苷酸磷酸黄递酶(NADPH-d)在三叉神经感觉核的神经元中有表达。切断咬肌神经诱导NADPH-d在三叉神经运动核和中脑核重新表达。N-NOS的表达与NADPH-d的表达相似，但延迟了约一周。观察钙结合蛋白、钙结合蛋白、钙视黄素和小白蛋白的免疫组织化学分布及NADPH-d的酶组织化学分布。较少

A member of DNA binding proteins, c-Fos was upregulated in neurons of the trigeminal sensory nucleus after electrical stimulation of the trigeminal ganglion. The expression was dependent on the intensify of the stimulation and the positive neurons were distributed in a nucleus specific manner. In the subnucleus caudalis (SpVc), most labeled neurons were in the superficial zone in its full extetent of the dorsomedial ventrolateral axis. In the oral subnucleus, c-Fos-positive neurons appeared only in the dorsomedial subnucleus. The c-Fos expression induced by a low intensity stimulation (0.1 mA), which activates A-beta fibers, and a high intensity stimulation (1.0 mA) which activates also C-fibers, was differently regulated by preadministration of GABAA and GABAB receptors agonist and antagonist. The segmental and supraspinal intensity-dependent GABA ergic regulation in the trigeminal sensory nucleus has been in plicated. Intrathecal administration of c-fos antisense oligodeoxynucleotide … More reduced adjuvant induced cFos expression and prolonged thermal hyperalgesia but not normal nociception. Thus c-Fos expression after nociception is involved in maintenance and/or development of hyperalgesia. Electrical stimulation of the somatosensory area of the cerebral cortex is applied in many patients suffering from intractable pain with good to excellent pain relief. We demonstrated that electrical stimulation of the secondary sensory area of the cortex significantly decreased the number of formalin-induced c-Fos expression in the SpVc. A neuronal nitric oxide synthase (n-NOS) and nicotinamide adenine dinucleotide phosohate-diaphorase (NADPH-d) is expressed in discrete population of neurons in the trigeminal sensory nucleus. Transection of masseteric nerve induced de novo expression of NADPH-d in the trigeminal motor nucleus and mesencephalic nucleus. The n-NOS expressed similarly with the NADPH-d expression, however delayed about one week. Immunohistochemical distribution of calcium binding proteins, calbindin, calretinin and parvalbumin and enzyme histochemical distribution of NADPH-d has been examined. Less