Change in expression of c-fos and c-jun gene family in carcinogenesis of the oral mucosa.

很难区分哪些癌前病变会变成恶性肿瘤。采用免疫组织化学方法，对口腔鳞癌、白斑、扁平苔藓、乳头状瘤等口腔病变的癌基因c-fos和c-jun的表达模式进行了研究。临床资料用10%福尔马林固定，石蜡包埋，切片5μ。用抗c-Fos和c-Jun抗体孵育后，同时用罗丹明标记的抗体和FITC标记的抗体对c-Fos和c-Jun进行染色，并在荧光显微镜下观察。C-Fos(-)/c-jun(+)和c-Fos(-)/c-jun(-).c-Fos(+)/c-jun(+)细胞分布于正常口腔黏膜、白斑和扁平苔藓皮损的基底层。C-Fos(+)/c-Jun(-)细胞在少数正常口腔黏膜中可见，而在有浸润性倾向的鳞癌中，部分白斑和扁平苔藓病变有向下生长的趋势。C-Fos(-)/c-jun(+)细胞在有丝分裂的鳞状细胞癌中呈散在分布，提示有c-Fos(+)/c-jun(-)细胞的白斑和苔藓病变有恶变倾向。

It is difficult to differentiate which precancerous lesions are going to malignant tumors. To differentiate the precancerous lesions which are going to malignant tumors from those which remain not to be malignant, we studied the expression pattern of oncogenes, c-fos and c-jun, immunohistochemically about various oral lesions : squamous cell carcinoma, leucoplakia, lichen planus, papilloma, etc.The clinical materials were fixed in 10% formalin, embedded in paraffin and cut in 5μ thickness by a microtome. After incubation with anti-c-Fos and c-Jun antibody, the sections were stained by rhodamine-conjugated IgG for c-Fos and by FITC-conjugated IgG for c-Jun at the same time, and observed by a fluorescent microscopy.Stainability of the epithelial lesions was divided into c-Fos(+)/c-Jun(+), c-Fos(+)/c-Jun(-), c-Fos(-)/c-Jun(+) and c-Fos(-)/c-Jun(-).c-Fos(+)/c-Jun(+) cells were observed beneath the keratinized layer and sporadically at the basal cell layer of the normal oral mucosa, leucoplakia and lichen planus lesions. C-Fos(+)/c-Jun(-) cells were detected in few cases of normal oral mucosa, but in the squamous cell carcinoma of infiltrative tendency, some parts of leucoplakia and lichen planus lesions with down growth tendency. C-Fos(-)/c-Jun(+) cells were observed scatteringly in the squamous cell carcinoma with frequent mitosis.From the above findings it may be suggested that the lesions of leucoplakia and lichen plamus with c-Fos(+)/c-Jun(-) cells have a tendency to change into malignancy.