A study of genetic risk factors for susccptibility to pcriodontal discascs by molccular biology

本研究的目的是阐明CD14、TNF-α和IL-1基因型之间的关系。牙周炎的状况和类型。以及巨噬细胞的功能。研究对象为55例成人牙周炎（AP）患者、35例早发性牙周炎（EOP）患者和40例健康对照者。采用PCR-RFLP法测定cd14、TNF-α、IL-1基因型。研究结果如下：IL-1A^<-889>等位基因2基因型在患者中的患病率高于对照组。EOF组与对照组比较差异有统计学意义。由IL- 1a ^<-889>等位基因2 + IL- 1b ^<+3953>等位基因2组成的复合基因型在患者组中患病率更高。EOF组与对照组比较差异有统计学意义。CD14、TNF-α - 4基因型分布在牙周炎患者和对照组、牙周炎类型之间均无差异。基因型与巨噬细胞功能（LPS刺激外周血单核细胞产生TNF-α、IL-1α、IL-1β、可溶性CD14）之间无相关性。流式细胞术分析cd14在巨噬细胞中的表达强度)。这些结果表明，IL-1基因型是日本人群牙周病（包括早发性牙周炎）的一个有用的风险标记。

The objectives of this study were to clarify the association of genotypes of CD14, TNF-α, and IL-1. And condition and types of pcriodontilis. And function of the macrophagcs.Fifty-five patients with adult pcriodonlitis (AP), 35 patients with early-onset periodontilis (EOP) and 40 healthy controls were participated in this study. Genotypes of CD 14, TNF-α, IL-1 were determined using PCR-RFLP method.The results as follows:1. The prevalence of the IL-1A^<-889> allele 2 genotype was greater in the patients than in controls. The difference between the EOF and the control groups was significant.2. The prevalence of the composite genotype comprising allele 2 of IL-1A^<-889> plus allclc 2 of IL IL-1B^<+3953> was greater in the patient group. The difference between the EOF and control groups was significant.3. No differences were observed between the patient and control groups, or types of periodontitis in distribution of genotypes of CD14, TNF-α.4. No association was observed between the genotypes and function of macrophagcs (production of TNF-α, IL-1α, IL-1β, soluble CD14 from peripheral-blood mononuclear cells stimulated with LPS. And expression intensity of CD 14 on macrophages analyzed using flow cytomelry).These results suggest that IL-1 genotype is a useful risk marker for periodonlal diseases including earlyonset periodontitis in Japanese population.