Morphological studies on neuronal and molecular features of sensory input-controls

1.大鼠延髓背角(MDH)内P物质(SP)的EM研究表明，轴突终末释放的P物质(SP)不仅作用于面对含SP轴突终末的NK1受体，而且扩散到细胞外液中，作用距离大于突触间隙，作用于距离突触部位一定距离的NK1受体。2.用双免疫荧光组织化学方法观察了大鼠延髓背角神经元中钙结合蛋白的共存情况。3.大鼠延髓背角中脑核神经元胞体与GABA能终末有突触接触，表达功能性BA_a>受体而不表达功能性GABA_<b>受体。在切片制作中，采用细胞内记录和生物细胞素注射结合组织化学和免疫组织化学染色技术，观察了大鼠中缝核团对MNT神经元的Seretonine能神经支配。4.含SP的初级传入纤维与大鼠MDH I、II层的GABA能和甘氨酸能神经元发生突触接触。6.大鼠中缝背核(DR)内的一些神经元通过轴突侧支同时投射到纹状体和中缝大核/巨细胞核α区。用细胞内记录和生物细胞素注射结合免疫组织化学的方法，在切片上观察了DR神经元的形态特征和电膜特性之间的对应关系。7.在大鼠中，蛋白激酶C-γ在许多伤害性相关的MDH神经元中表达。

1.The EM study in the rat medullary dorsal horn (MDH) suggested that substance P (SP) released from axon terminals might not only act on NK1 receptors facing SP-containing axon terminals but also diffuse in the extracellular fluid for distances larger than the synaptic cleft to act NK1 receptors at some distances from the synaptic sites.2.Co-existence of calcium-binding protein in neurons of the rat medullary dorsal horn was examined by double immunofluorescence histochernistry.3.Neuronal cell bodies in the mesencephalic nucleus of teigeminal nerve (MNT) of the rat were indicated to be in synaptic contact with GABAergic axon terminals and to express functional GABA_<A>receptors but not functional GABA_<B>receptors. Seretoninergic innervation of MNT neurons by the raphe nuclei were also observed.4.SP-containing primary afferent fibers were found to be in synaptic contact with GABAergic and glycinergic neurons in laminae I and II of the rat MDH.5.In slice perparations, correspondence between morphological features and electrical membrane properties of rat MDH neurons were examined in slice preparations by intracellular recording and biocytin-injection combined with histochemical and immunohistochemical staining techniques. The intrinsic MDH neurons were classified into 4 groups.6.Some neurons in the rat dorsal raphe nucleus (DR) were found to project simultaneously to the striatum and the raphe magnus nucleus/gigantocellular nucleus alpha region by axon collaterals. Correspondence between morphological features and electrical membrane propeties of DR neurons were also examined in slice perparations by intracellular recording and biocytin-injection combined with imrnunohistochemistry.7.It was indicated in the rat that protein kinase C gamma was expressed in many nociception-related MDH neurons.