Cytogenetically analysis of congenital abnormality and childhood malignancy using multicolor FISH methods

我们建立了彩色条带FISH法进行精确的细胞遗传学分析，并于2001年开始临床样本采集。我们正在测试将临床样本转移给合作者Michael speech教授的方法，以便在慕尼黑对这些临床样本进行多色FISH分析。儿童白血病的临床样本来自“日本儿童白血病研究协会，J*****”。为了将彩色条带FISH法与常规FISH法相结合的新方法检测特定基因，我们对一些候选基因进行了功能分析。其中，我们发现B细胞特异性转录调节因子BACH2在氧化应激下诱导细胞凋亡。此外，我们还发现Bach2不仅在B细胞中表达，而且在脑中也表达。我们与德国慕尼黑的Thomas Cremer教授合作建立了活细胞中染色体动力学的研究方法来研究染色体易位的机制，并利用该方法研究染色体区域的动力学。

We established Color banding FISH method for the precise cytogenetical analysis, and started collection of clinical sample in 2001.We are testing the ways to transfer clinical sample to Prof. Michael Speeches, a collaborator, for multicolor FISH analysis of these clinical samples in Munich. Clinical samples of childhood leukemia are provided from "Japan Association of Childhood Leukemia Study, J*****.To identify new genes associated with childhood leukemia by the new method combining color banding FISH method and conventional FISH method to detect a specific gene, we have done functional analysis of some candidate genes. Among them, we have revealed that BACH2, a B cell specific transcription regulator, showed induction of apoptosis upon oxidative stress. Furthermore, we also found that Bach2 is expressed not only in B cell but also in brain.We have established a method to study the dynamics of chromosome in living cell to study the mechanism of chromosome translocation with Prof. Thomas Cremer, a Collaborator in Munich, and study the dynamics of chromosome territories using method.