Detection of Doxirubicin Induced Mycardial cell injury using Monoclonal Antibodies for Myocardial Annexin V

应用心肌膜联蛋白单克隆抗体检测多西他赛诱导的心肌细胞损伤VAnnex in V是一种存在于心肌可溶性部分的钙依赖性磷脂结合蛋白，当充血性心力衰竭患者的心肌受损时，Annex in V从心肌可溶性部分移位至心肌细胞膜。本研究的目的是阐明In-111标记的心肌膜联蛋白V单克隆抗体对心肌损伤的诊断价值。方法. Wister大鼠40只，每周注射多西泮0.2mg，制作多西泮心肌病模型。在注射多西鲁肽后4、6、8和9周注射In-111标记的抗Annexin V抗体。结果多昔洛韦组第4、6、8、9周的心/肺比值（2.56±0.48、2.30±0.49、2.20±0.23、0.85±0.41）明显高于对照组（1.30±0.21、1.32±0.38、1.39±0.23）。生物分布分析还显示，In-111标记的抗Annexin V抗体在阿霉素组的蓄积量高于对照组。结论In-111标记的抗Annexin V抗体在阿霉素诱导的大鼠心肌病模型中阳性成像受损心肌。

Detection of Doxirubicin Induced Mycardial cell injury using Monoclonal Antibodies for Myocardial Annexin VAnnex in V, a calcium-dependent phospholipid-binding protein that exists in the soluble fraction of myocardium, was translocated from the soluble fraction of myocardium to the myocardial cell membrane when the myocardium was damaged in the patients with congestive heart failure. The purpose of this sturdy was to clarify the diagnostic value of In-111 labeled Monoclonal Antibodies for Myocardial Annexin V for the detection of damaged myocardium. Methods. Forty Wister rats were injected 0.2mg of doxirubicin weekly for making doxirubicin induced cardiomyopathy model. In-111 labeled anti-Annexin V antibody was injected 4, 6, 8 and 9 weeks after the doxirubicin Injection. Results. Heart/Lung ratio in doxirubicin group on weeks 4, 6, 8 and 9 weeks (2.56±0.48, 2.30±0.49, 2.20±0.23, 0.85±0.41) were significantly higher than that in control group (1.30±0.21, 1.32±0.38, 1.39±0.23). Biodistribution analysis also showed that In-111 labeled anti-Annexin V antibody accumulation was higher in doxirubicin group than that in control group. Conclusion. In-111 labeled anti-Annexin V antibody scintigraphy positively imaged damaged myocardium in doxirubicin induced rat cardiomyopathy model.