A study of fiber connections controlling jaw movement and salivary secretion with the use of mmnohistochemical and in situ hybridization technique

GABA和甘氨酸被认为是主要的抑制性神经递质。本研究旨在证实GABA_A受体样免疫反应（R-LI）、GABA_B-R-LI和甘氨酸-R-LI谱在大鼠出生后发育过程中在三叉神经运动核（Vm）中的共定位。实验对象为0 ~ 35日龄雌性Wistar大鼠。采用共聚焦激光扫描显微镜和电子能谱衍射显微镜对GABA-R-LI和Gly-R-LI谱进行免疫细胞化学检测。7天大时，Gly-R-LI谱聚集在Vm的背外侧。在胞体细胞膜和Vm运动神经元近端树突上可见，部分细胞体也有GABA_B-R-LI标记。28 ~ 35日龄时，Gly-R-LI在Vm内几乎消失，而GABA_A-R-LI和GABA_B-R-LI在Vm神经元胞体的几乎所有运动神经元上仍可见。这些结果表明，抑制性神经递质受体在哺乳到咬食行为的过渡阶段改变了它们的分布。

GABA and glycine are thought to be major inhibitory neurotransmitters. This study was undertaken to demonstrate co-localization of GABA_A receptor-like immunoreactive (R-LI), GABA_B-R-LI and glycine-R-LI profiles in the rat trigeminal motor nucleus (Vm) during the course of their postnatal development. Experiments were carried out on female Wistar rats of 0 to 35 days old. GABA-R-LI and Gly-R-LI profiles were detected immunocytochemically with the use confocal laser scanning microscope and electron spectroscopic diffraction microscope. At 7 days old Gly-R-LI profiles were aggregated in the dorsolateral part of Vm. They were seen on the cell membrane of the somata and the proximal dendrites of a Vm motoneuron, and some cell bodies were also labeled with GABA_B-R-LI. In 28 to 35 days old animals Gly-R-LI was almost disappeared in the confine of Vm but GABA_A-R-LI and GABA_B-R-LI were still observed on almost all motoneurons of Vm neuronal cell bodies. These results suggest that receptors for inhibitory neurotransmitters shift their distribution in the transition phase of suckling to biting behavior of the rat.