THE INFLUENCE OF PH AND THE ELUCIDATION OF CONTAMINATION ROUTE FOR LEGIONELLA IN HOT SPRING WATERS

1.对不同温泉浴场室内外水样氯化后水质的变化进行了分析，并对军团菌进行了流行病学研究。2.在不同的pH和温度条件下，考察了不同pH和温度条件下微宇宙中嗜肺链球菌种群向VNC(活的但不可培养)阶段的转变。(1)在极低pH的微宇宙中，嗜肺链球菌的整个细胞在短时间内被杀死，而在高pH的微宇宙中，细胞数量逐渐减少。另一方面，提示PH5的微宇宙中的细胞群向VNC阶段转移。(2)嗜肺乳杆菌可在pH值为6.0~8.2或2 5~42℃的微宇宙中长期存活。(3)以前认为嗜肺乳杆菌不能在45℃的高温水中存活，但目前的资料表明，即使在此条件下，嗜肺乳杆菌仍可作为VNC存活。然而，嗜肺性乳杆菌是Q…3.检测了氯对嗜肺乳杆菌和棘阿米巴的防腐效果。(1)嗜肺乳杆菌在微宇宙中可存活1分钟以上，余氯0.4ppm。在1.0ppm的剩余氯中，这些微生物很快就被杀死了。(2)将棘阿米巴细胞加入到小宇宙中，然后在阿米巴细胞的吞噬小体中繁殖并吞噬细胞，并在孵育过程中重复上述过程。孵育24小时后，在微宇宙中加入不同浓度的氯。即使在用50ppm余氯消毒的微型宇宙中也能检测到少量的肺炎链球菌。40%的棘阿米巴细胞生活在剩余氯为10ppm的微型宇宙中。4.研究了军团菌污染SpA的途径。(1)采用随机扩增多态性DNA分析技术，对SpA和周围土壤中分离到的血清型相同的嗜肺乳杆菌菌株进行了基因分型。到目前为止，这些分离株之间的遗传同一性尚未得到承认。(2)对从带循环系统的温泉浴缸和滤池中分离到的不同血清型嗜肺乳杆菌分离株进行了随机扩增多态性DNA分析。不同血清型的分离株之间存在遗传一致性。进一步用脉冲场凝胶电泳法(PFGE)进行验证。较少

1.Change in the quality due to chloridizing against sampled water collected from indoor or outdoor bathtub of various spa was analyzed and epidemiological study of Legionella spp. in these water was carried out.2.Transition to VNC (viable but not cultivable) stage of L.pneumophila population in microcosms preparated in sampled water was examined under conditions of different pH and temperature(1)Whole cells of L.pneumophila were killed in a short period in microcosm with extreme low pH, while cell numbers were gradually reduced in microcosm with high pH. On the other hand, it was suggested that cell population in microcosm with pH5 shifted to VNC stage. (2)L.pneumophila could survive for a long period in microcosms in a range of pH6.0 to 8.2 or 25 to 42℃ (3)It has previously been thought that L.pneumophila could not survive in high-temperature water of 45℃ however, the present data suggested that L.pneumophila could survive as VNC even under this condition. However, L.pneumophila was q … More uickly killed in microcosms with temperature of 50 or more degrees Celsius.3.Antiseptic effect of chlorine against L.pneumophila and Acanthamoeba was examined.(1)L.pneumophila could survive for one minute or more in microcosm with 0.4 ppm of residual chlorine. In residual chlorine of 1.0 ppm, the organisms were quickly killed. (2)Acanthamoeba cells were added into microcosms, and then L.pneumophila multiplicated in phagosomes of amoeba cells and ate away cells, and repeated these process during incubation. After incubation for 24 hrs, a various concentrations of chlorine were added into microcosms. A small amount of L.pneumonia cells was detected even in microcosm disinfected with 50 ppm of residual chlorine. Forty % of Acanthamoeba cells was living in microcosm with 10 ppm of residual chlorine. These cells might exsist as cyst cell having resistance to chlorine, therefore, L.pneumonia cells were also protected in these cysts from bactericidal effect of chlorine.4.The route that Legionella contaminate spa was studied.(1)Genotype of L.pneumophila isolates with identical serotype isolated from spa and the surrounding soil was determined by randomly amplified polymorphic DNA analysis(RAPD). As so far examined, genetic identity among these isoltes was not recognized. (2)RAPD analysis was done about L.pneumophila isolates of a variety of serotype isolated from bathtub and filtlate tank in spa with circulating system. Genetic identity was shown among isolates of different serotype. Further confirmation by pulse field gell electrophoresis(PFGE) was carried out. Less