Signal transduction of GPI-anchored proteins in lipid rafts

GPI锚定的神经细胞黏附分子的信号转导机制尚不清楚，因为GPI锚定的蛋白没有跨膜区。最近的研究表明，糖鞘糖脂(脂筏)的动态聚集在信号转导过程中作为连接GPI锚定蛋白和src家族蛋白的平台。在这项研究中，我探索了GPI锚定的神经细胞黏附分子的信号转导机制。我以前报道过，GPI锚定的神经细胞黏附分子Tag-1通过src家族激酶Lyn在大鼠小脑原代培养的脂筏中传递信号。在这一年里，我发现TAG-1配体磷酸聚糖通过src家族激酶Lyn在Lipis筏中诱导信号转导，并对大鼠原代小脑培养产生排斥作用。

The mechanism of signal transduction by GPI-anchored neural cell adhesion molecules is obscure, because GPI-anchored proteins have no transmembrane domains. Recent studies suggest dynamic clustering of glycosphingolipids (lipid rafts) function as platforms for attachment of GPI-anchored proteins, src-family kinases during signal transduction. In this research project, I pursued clarifying mechanism of signal transduction by GPI-anchored neural cell adhesion molecules.I previously reported that GPI-anchored neural cell adhesion molecule TAG-1 transduces signal via src-family kinase Lyn in lipid rafts of rat primary cerebellar cultures. In this year, I found that phosphacan, TAG-1 ligand, induces signal transduction in lipis rafts via src-family kinase Lyn and has repulsive effects on rat primary cerebellar cultures.