Exhausitive search for the identification of lung cancer specific gene splicing by microarray technique

随着CT检查变得流行，周围型小肺腺癌在这些日子里被更频繁地发现。周围型小肺腺癌CT M/L比值(纵隔CT图像肿瘤面积/肺CT图像肿瘤面积)与患者预后相关。实体型组(CT M/L比值50%)Ki-67高标记指数及血管内皮生长因子、CD34、基质金属蛋白2和基质金属蛋白酶9高表达患者数显著高于淡密度组(CT M/L比值50%)。淡密度型组CD44v6或基质金属蛋白酶组织抑制因子2高表达的患者数明显高于实质型组。可预测CT M/L比值的独立变量有CD34、基质金属蛋白酶2、基质金属蛋白酶9和基质金属蛋白酶组织抑制因子2。使用新开发的启动子相关甲基化DNA扩增DNA芯片对基因启动子区域的DNA甲基化进行微阵列分析。在肺癌细胞系中，大约10.9%的癌症相关基因发生了高甲基化。值得注意的是，在所研究的大多数肺癌细胞系中，HIC1、IRF7、ASC、RIPK3、RASSF1A、FABP3、PRKCDBP和PAX3基因高甲基化。这些基因的表达谱与基因的甲基化谱相关，表明基因启动子区DNA甲基化的微阵列分析为表观遗传学研究提供了便利。

Peripheral small lung adenocarcinomas have been detected more frequently in these days according as CT screening has become popular. The computed tomographic M/L ratio (area of the tumor in the mediastinal computed tomographic image/area of the tumor in the lung computed tomographic image) of small peripheral lung adenocarcinoma is correlated with patient prognosis. The numbers of patients with high labeling indexes of Ki-67 and high expression of vascular endothelial growth factor, CD34, matrix metalloproteinase2, and matrix metalloproteinase9 in the solid-type group (computed tomographic M/L ratio >50%) are significantly higher than those in the faint density-group (computed tomographic M/L ratio <50%). The number of patients with high levels of CD44v6 or tissue inhibitor of matrix metalloproteinase2 staining in the faint density-type group is significantly higher than that in the solid-type group. Independent variables capable of predicting computed tomographic M/L ratio included CD34, matric metalloproteinase 2, matrix metalloproteinase 9, and tissue inhibitor of matrix metalloproteinase 2.Aberrant methylation of DNA is an important event in carcinogenesis. A microarrray analysis of DNA methylation in the promoter regions of genes using a newly developed promoter-associated methylated DNA amplification DNA chip. About 10.9% of the cancer-related genes are hypermethylated in lung cancer cell lines. Notably, HIC1, IRF7, ASC, RIPK3, RASSF1A, FABP3, PRKCDBP, and PAX3 genes are hypermethylated in most lung cancer cell lines examined. The expression profiles of these genes correlated to the methylation profiles of the genes, indicating that the microarray analysis of DNA methylation in the promoter region of the genes is convenient for epigenetic study.