Application of gel DNA/artificial lipid complex for new scaffolds in tissue regeneration

以l -丙氨酸为原料，与聚亚甲基糖醇（PMG）或聚乙二醇（PEG）反应合成了人工脂质。通过DNA与阳离子脂质反应制备凝胶DNA/脂质复合物。虽然它们的细胞毒性很低，不会引起不良的组织反应，但它们在PBS缓冲液和大鼠皮下组织中降解的时间相对较短。发现DNA/指示性脂质复合物不适合作为诱导新骨发育的支架。因此，由于壳聚糖等含DNA复合物在一个分子中具有多个官能团，因此可以通过DNA与多阳离子交联来控制其在体内或体外的溶解速率。DNA/壳聚糖凝胶复合物在体内和体外的作用都比DNA/脂质复合物慢，比DNA/脂质复合物更适合作为支架。采用新型PBS缓冲溶液法在模具中制备了含和不含磷灰石的DNA/壳聚糖凝胶复合物。DNA/壳聚糖凝胶配合磷灰石可诱导新骨发育。此外，经阳离子脂质处理的DNA/壳聚糖凝胶复合物具有抗真菌活性，有望用于GTR的研究。DNA/壳聚糖凝胶复合物在加压后也成为透明薄膜。该薄膜可用于医用敷料和粘附屏障。

The artificial dicationic lipids were synthesized from the reaction of L-alanine, and polymethylene glycohols (PMG) or polyethylene glycohols(PEG). Gel DNA/lipid complexes were prepared by reaction of DNA with cationic lipids. Although they were very low cytotoxic and do not cause an unfavorable tissue response, they were degraded relatively short periods in PBS buffer solution, and in the subcutaneous tissues of rats. It was found that DNA/dication lipid complexes were not suitable for scaffolds, which can induce development of new bone. Therefore, it predicted that the rate at which DNA-containing complexes dissolved in vivo or vitro can be controlled by crosslinking DNA with polycation such as chitosan because they have many functional groups in one molecule. The DNA/chitosan gel complexes were regarded in vivo or in vitro more slowly that DNA/dication lipid complexes and were more suitable for scaffolds than DNA/dication lipid complexes. The DNA/chitosan gel complexes with and without apatite were fabricated in mold by newly PBS buffer solution methods. The DNA/chitosan gel complexes with apatite induced induce development of new bone. Moreover, the DNA/chitosan gel complex treated with cation lipid had antifungal activity, and they will be useful for GTR. The DNA/chitosan gel complex also became transparent film after being pressed. This film will be useful for medical dressing materials and an adhesion barrier.