A genomic and phenomic investigation of a mitochondrial glutathione transferase

本研究旨在了解线粒体中新发现的谷胱甘肽转移酶Kappa(GSTK)的基因组学、结构和功能。调查将取得几个结果。(1)了解GSTK基因在人和小鼠中的组织结构(S)；(2)通过研究基因敲除小鼠的表型，确定GSTK在线粒体中的作用；(3)确定人类GSTK的晶体结构；(4)了解GSTK的底物专一性、反应动力学和结构/功能关系。由于GSTK仅限于线粒体，可能与其他GSTs无关，我们也可能发现新的功能

The aim of this study is to understand of the genomics, structure and function of glutathione transferase Kappa (GSTK), a novel GST found in mitochondria. The investigations will achieve several outcomes. (1)an understanding of the organisation of GSTK gene(s) in humans and mice; (2) determination of the role of GSTK in mitochondria, by investigating the phenotype of knockout mice; (3) determination of the crystal structure of human GSTK; (4) An understanding of GSTK's substrate specificity, reaction kinetics and structure/function relationships. Since GSTK is confined to mitochondria, and may not be related to other GSTs, we may also identify novel functions